远志筒与根的HPLC指纹图谱及化学差异性分析

HPLC Fingerprint and Active Components Determination of Polygala tenuifolia Root Bark and Root

目的:对远志筒与根的HPLC指纹图谱及化学差异性进行分析。方法:对不同批次远志药材样品进行细叶远志皂苷、远志酮Ⅲ和3,6'-二芥子酰基蔗糖的含量测定,优化现有指纹图谱方法,对其进行相似度分析并结合多元统计分析进行综合比较。结果:不同批次远志样品间指纹图谱的相似度较高(均大于0.9),聚类分析与主成分分析发现,远志筒与根可以明显区分,而远志酮Ⅲ与3,6'-二芥子酰基蔗糖含量的高低对分类影响较大。散点图结果显示,不同批次远志药材样品间细叶远志皂苷的含量差异较大;筒中远志酮Ⅲ、3,6'-二芥子酰基蔗糖的含量变化范围较窄,而根中远志酮Ⅲ含量变化范围较大。堆积图结果表明,不能单以某种成分含量的高低来评价药材质量。结论:远志筒与根中3,6'-二芥子酰基蔗糖的含量差异较大。今后应根据临床的不同治疗作用,选择远志不同部位入药,提高临床用药的准确性。

Objective： To compare the difference between Polygala tenuifolia root bark and root using HPLC fingerprint analysis and active components determination. Methods： Three main characteristic components including tenuifolin,polygalaxanthone Ⅲ,and 3,6＇-disinapoly sucrose in different Polygala tenuifolia samples were determined by HPLC. The fingerprint methods were optimized. Then,the similarity evaluation and multivariate statistical analysis were applied in the post-processing data analysis. Results： There was a high similarity in 15 batches of Polygala tenuifolia samples and its similarity coefficient was greater than 0. 9. The Polygala tenuifolia root bark and root could be clearly separated by the cluster analysis and / or the principal component analysis due to significant differences of the contents of polygalaxanthone Ⅲ and 3,6＇-disinapoly sucrose. In addition,the result of scatter plot showed that there was a high variation of tenuifolin content among all samples. The contents range of polygalaxanthone Ⅲ and 3,6＇-disinapoly sucrose in root bark varied relatively slight,while the content range of polygalaxanthone Ⅲ in roots varied largely. Moreover,the stacked plots suggested that it was not reasonable to evaluate the quality of Polygala tenuifolia only by a single ingredient. Conclusion： The content of 3,6＇-disinapoly sucrose varies more largely than the other two active components in Polygala tenuifolia root bark and root. Polygala tenuifolia root bark and root should be selected for different clinical treatment to ensure the accuracy of its clinical use.