蔓性千斤拔与大叶千斤拔的鉴别研究

Identification of Moghania philippinensis and Moghania macrophylla

目的:对蔓性千斤拔及大叶千斤拔进行鉴别研究,建立其全面、准确的鉴别方法。方法:采用薄层色谱法和高效液相色谱法对两种千斤拔干燥根中的染料木苷进行定性定量分析,通过DNA分子鉴定技术建立其DNA条形码。结果:在TLC的基础上,运用HPLC建立了检测千斤拔药材中染料木苷含量的方法;建立了基于ITS2序列的蔓性千斤拔与大叶千斤拔的DNA条形码,从分子水平上将二者区分。结论:运用不同的鉴定方法可对蔓性千斤拔及大叶千斤拔进行准确、全面、快速的鉴定,避免了因为方法的局限性影响鉴别的准确性,为千斤拔药材的鉴别提供新的科学依据。

Objective： To study the identification methods of Moghania philippinensis and Moghania macrophylla,and to establish a comprehensive precise discrimination method. Methods： TLC and HPLC were applied to analyze genistein in the root of Moghania philippinensis and Moghania macrophylla. DNA barcoding establishment was based on ITS2 sequcence. Results： A comprehensive differentiation method for Moghania philippinensis and Moghania macrophylla based on TLC was proposed,which was combined with HPLC for determination of genistein. The plants of Moghania philippinensis and Moghania macrophylla and their related species could be distinguished by DNA barcoding effectively. Conclusion： TLC and HPLC profiles of Flemingia Radix provide alternative methods of identification using chemical approach. This integrated chemical and molecular approach allows accurate comprehensive fast identification of Moghania philippinensis and Moghania macrophylla,which avoids the methods limitations on the accuracy of identification. The differentiation methods based on TLC,HPLC and DNA barcoding are simple,which provide a new scientific evidence for the identification of authenticity of Flemingia Radix.