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超高效液相色谱-串联质谱快速测定饮料中甜蜜素的方法探讨 被引量:1

Rapid determination of sodium cyclamate in beverage by ultra high performance liquid chromatography-tandem mass spectrometry
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摘要 目的建立超高效液相色谱-串联质谱法快速测定饮料中甜蜜素的方法。方法样品经Agilent Eclipse plus C18色谱柱分离,流动相为乙腈-乙酸铵溶液(0.02 mol/L),电喷雾串联质谱多反应监测模式测定,外标法定量。结果甜蜜素在测定的范围内线性关系良好(r=0.9990),方法的检出限为0.2μg/L(3S/N)。在加标水平为1、10、100μg/L时,方法的回收率为94.3%~110%,相对标准偏差(n=6)为0.29%~2.42%。结论该方法对样品无需衍生化,前处理简单,分析时间短,选择性好,灵敏度高,适用于甜蜜素的快速定性定量检测。 [ Objective ] To develop a new analytical method for the rapid determination of sodium cyclamate in beverage by ultra high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). [Methods] The samples were separated on an Agilent Eclipse plus C18 column and analyzed by LC-MS/MS under muhiple reaction monitoring mode via positive eleetrospray ionization, with the mobile phase of acetonitrile-ammonium acetate solution (0.02 tool/L). The quantitation analysis was carried out by the matrix-matched external standard calibrations. [ Results ] The method showed excellent linearities (r=0.999) for the sodium cyclamate in a certain concentration range. The detection limit was 0.2 p^g/L (3S/N). The recoveries at three added standard levels of 1, 10, and 100 Ixg/L were between 94.3% and 110%, and the relative standard deviations (n =6) were 0.29%-2.42%. [Conclusion] The method has the advantages of sample without derivatization, simple pretreatment, short analysis time, good selectivity, high sensitivity, which is suitable for rapid qualitative and quantitative determination of sodium cyclamate.
出处 《职业与健康》 CAS 2015年第18期2470-2472,共3页 Occupation and Health
关键词 超高效液相色谱-串联质谱 甜蜜素 饮料 Ultra high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) Sodium cyclamate Beverage
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