摘要
The building block of eukaryotic chromatin is the nucleo- some core particle （NCP）, which is consisted of -146 bps of DNA wrapped around an octamer of core histones. A tetra- mer of histone H3 and H4 and two H2A-H2B dimers form the histone octamer （Kornberg, 1974; Luger et al., 1997; Tho- mas and Kornberg, 1975）. During DNA replication, nucleo- some disassembly and reassembly occurs at the replication fork, and histone chaperons CAF-1 and ASF1 are principally responsible for the deposition of histones H3 and H4 onto replicated DNA （Kaufman et al., 1995; Tyler et al., 1999）. ASF1 binds a heterodimeric histone H3-H4 complex through its conserved N-terminal domain （aa 1-155） and impedes the formation of the （H3-H4）2 tetramer both inside the nucleus and in the cytoplasm （English et al., 2005）.
The building block of eukaryotic chromatin is the nucleo- some core particle （NCP）, which is consisted of -146 bps of DNA wrapped around an octamer of core histones. A tetra- mer of histone H3 and H4 and two H2A-H2B dimers form the histone octamer （Kornberg, 1974; Luger et al., 1997; Tho- mas and Kornberg, 1975）. During DNA replication, nucleo- some disassembly and reassembly occurs at the replication fork, and histone chaperons CAF-1 and ASF1 are principally responsible for the deposition of histones H3 and H4 onto replicated DNA （Kaufman et al., 1995; Tyler et al., 1999）. ASF1 binds a heterodimeric histone H3-H4 complex through its conserved N-terminal domain （aa 1-155） and impedes the formation of the （H3-H4）2 tetramer both inside the nucleus and in the cytoplasm （English et al., 2005）.
