心脑宁胶囊对高尿酸血症大鼠心肌细胞炎症反应及血清尿酸、C反应蛋白的影响

Effect on the Myocardial Cell Inflammatory Response,Serum Uric Acid,C Reactive Protein of Rats with Hyperuricemia by Xinnaoning Capsules

目的:观察心脑宁胶囊对高尿酸血症大鼠心肌细胞炎症反应,血清尿酸、C反应蛋白(C-reactin protein,CRP)变化的影响。方法:60只大鼠随机分为正常组、实验组和对照组。正常组给予质量分数0.5%羧甲基纤维素钠,25 mg·kg-1;实验组给予质量分数0.5%羧甲基纤维素钠,25 mg·kg-1,氧嗪酸钾1.5 g·kg-1,心脑宁胶囊1.8 g·kg-1;对照组给予质量分数0.5%羧甲基纤维素钠,25 mg·kg-1,氧嗪酸钾1.5 g·kg-1。均为灌胃给药,每日灌服1次,第30天,股动脉取血处死,取心肌,光镜下观察大鼠心肌细胞形态。测定血清尿酸、CRP水平。结果:用药后实验组、对照组血尿酸水平较正常组均明显升高(P<0.001);用药后实验组与对照组血尿酸水平比较,差异有统计学意义(P<0.05)。用药后实验组CRP水平与正常组比较,差异无统计学意义(P>0.05);用药后对照组CRP水平较正常组明显升高(P<0.001)。光镜下观察大鼠心肌细胞形态,实验组较对照组心肌细胞炎症反应明显减轻。结论:心脑宁胶囊可降低高尿酸血症大鼠心肌细胞炎症反应程度,降低CRP水平。

Objective： To observe the effect on the myocardial cell inflammatory response,serum uric acid,C reactive protein of rats with hyperuricemia by Xinnaoning capsules. Methods： 60 rats were divided into 3 groups： control group（ 0. 5% sodium carboxymethyl cellulose 25 mg·kg- 1）,group 1（ 0. 5% sodium carboxymethyl cellulose 25 mg·kg- 1Potassium Oxonate1. 5 g·kg- 1,Xinnaoning 1. 8g·kg- 1） and group 2（ 0. 5% sodium carboxymethyl cellulose 25 mg·kg- 1Potassium Oxonate 1. 5 g·kg- 1）. All the drugs were administered orally,1 times a day,for thirty days. Then all 3 groups of rats were executed and the Myocardium and blood was collected.The morphology of myocardial cells was observed under light microscope. Serum uric acid,CRP levels were measured and observed. Results： after treatment the serum uric acid levels of group 1 and group 2 were significantly increased（ P〈0. 001） compared with control group,and there was obvious difference about the serum uric acid level between group 1 and group 2（ P〈0. 05）; about the level of CRP,group 1 seemed roughly the same as the control group after treatment（ P〈0. 05）,whereas the level of CRP in group 2 was significantly increased compared with the control group（ P〈0. 001）. Under light microscope,we found that the inflammatory reaction was lightened significantly in groups 1 in contrast to group 2. Conclusion： Xinnaoning capsules can decrease the inflammatory reaction of rats with hyperuricemia and decrease the level of CRP.