红外三级鉴定法快速鉴别山豆根和北豆根

Identification of Radix Sophorae Tonkinensis and Rhizoma Menispermi by Three-Step Infrared Maro-Fingerprint Method

采用红外光谱三级鉴定法对山豆根和北豆根进行了鉴别研究。结果表明:山豆根和北豆根均含有饱和脂肪酸酯、芳香类化合物、黄酮类化合物和糖苷类化合物等物质。在一维红外光谱中,山豆根1 736、1 249cm-1和1 614、1 514、1 424cm-1峰的相对强度均比北豆根弱,说明山豆根中饱和脂肪酸酯成分、芳香类物质的含量低于北豆根。在高分辨率的二阶导数谱中,根据两者的差异可进一步发现山豆根和北豆根所含的饱和脂肪酸酯类成分存在差异,且北豆根中的芳香类物质含量比山豆根的高,而山豆根中淀粉的相对含量则高于北豆根。而二维相关红外光谱中,根据两者自动峰数目、位置和相对峰强度的差异,可进一步推断山豆根和北豆根所含的黄酮成分、糖苷类化合物是不一致的。因此,红外光谱三级鉴定法可为中药真伪鉴别提供一种快速、无损、整体、客观的方法。

Radix Sophorae Tonkinensis and Rhizoma Menispermihave have been studied by multi-stepinfrared macro-fingerprint method.The results showed that esters,aromatics,ketones and glycosides were commonly contained in Radix Sophorae Tonkinensis and Rhizoma Menispermi.Compared with FTIR spectra of samples,the stronger peaks around 1 736,1 249cm-1 and 1 614,1 514,1 424cm-1in FTIR spectra of Radix Sophorae Tonkinensis indicated the content of esters,aromatics were lower than Rhizoma Menispermi.Generally,the second derivative IR spectra can clearly enhance the spectra resolution.In the second derivative IR spectra,the position and sharp of characteristics peaks were very different.It＇s proved that the esters of them were different.Based on the difference of the relative peak intensity of the peaks,we could identify that the contents of aromatics in Rhizoma Menispermi were higher than Radix Sophorae Tonkinensis,and the content of starch in Radix Sophorae Tonkinensis was higher.In the 2D-IR spectra,they presented the differences in the numbers,positions and relative intensity of the autopeaks indicated that ketones and glycoside compounds of them were different.Therefore,the three-step IR macro-fingerprint provides a rapid,non-destructive comprehensive and objective method for the identification of traditional Chinese medicine.