纳米银-活性炭纤维敷料促进大鼠压疮创面愈合

Nano-silver active carbon fiber dressings improving bedsore wound healing in rats

目的研究纳米银-活性炭纤维敷料对压疮动物模型创面愈合情况的影响。方法选择成年SD大鼠,在背部制作压疮,根据创面覆盖和治疗材料的不同分为5组(n=10),分别为纳米银-活性炭纤维敷料组、纳米银敷料组、活性炭敷料组、洗必泰凡士林敷料组、空白对照组。比较5组大鼠的创面愈合情况、创面组织中血管新生因子和炎性因子的含量。结果大鼠的创面愈合时间和愈合率在5组间均有差异,纳米银-活性炭纤维敷料组大鼠的创面愈合时间短于其余4组,差异有统计学意义(P<0.05);压疮后第3、7、14、21d时,纳米银-活性炭纤维敷料组大鼠的愈合率均高于其余4组,差异均有统计学意义(P<0.05)。大鼠创面组织中的血管新生因子和炎性因子的含量在5组间有差异,纳米银-活性炭纤维敷料组大鼠创面组织中VEGFA、VEGFB、VEGFC的mRNA含量均高于其余4组,TNF-α、IL-2、IL-8的mRNA含量低于其余4组,差异均有统计学意义(P<0.05)。结论纳米银-活性炭纤维敷料有助于促进压疮创面愈合、缓解创面炎症反应、促进创面内新生血管形成,是处理压疮创面的理想材料。

Objective To study the effect of nano-silver active carbon fiber dressings on wound healing in bedsore wound animal models.Methods Adult SD rats were used and bedsore wound models were made on their back;the animals were then randomly divided into 5groups（n=10）according to different materials of the wound dressing and treatment.Groups were treated with nano-silver active carbon dressings,nano-silver dressings,active carbon dressings,Vaseline chlorhexidine dressings,or blank control.The wound healing situation,contents of angiogenic factors and inflammatory factors in wound tissues were compared among the 5groups.Results Wound healing time and the healing rates were different among the 5groups,with the healing time of rats in nano-silver active carbon dressing group being significantly shorter than those in the other 4groups（P〈0.05）.On the 3rd,7th,14 th and 21 st days after bedsore wound modeling,the healing rates of nano-silver active carbon dressing group were significantly higher than those of the other 4groups（P〈0.05）.The levels of angiogenesis factors and inflammatory factors were different in wound tissues of five groups,with the contents of VEGFA,VEGFB and VEGFC mRNA in nano-silver active carbon dressing group being significantly higher and the contents of TNF-α,IL-2and IL-8mRNA being significantly lower than those of the other 4groups（P〈0.05）.Conclusion The prepared nano-silver active carbon fiber dressing can help to improve pressure ulcer wound healing,relieve wound inflammation,and promote angiogenesis in the wound;it may serve as an ideal material for treating bedsore wound.