摘要
目的采用高效液相色谱法考察缬沙坦分子印迹聚合物的分子识别能力。方法制备缬沙坦分子印迹聚合物色谱柱;考察在不同的流动相中分子印迹聚合物对缬沙坦的分子识别能力及在相同流动相中聚合物对缬沙坦和其他化合物的色谱表征。结果以甲醇为流动相,缬沙坦分子印迹聚合物对缬沙坦表现出非常强的亲和力,有很好的印迹效率;对绿原酸也有很好的印迹效果。容量因子值随流动相中极性溶剂含量的增加而减小,以甲醇-醋酸(7∶3)为流动相时,聚合物对缬沙坦的印迹效应消失。结论缬沙坦分子印迹聚合物对缬沙坦具有良好的选择性,对绿原酸也有很好的印迹作用,因此可用于筛选中药提取物中与缬沙坦大小相近、结构或功能基团相似的化合物。
OBJECTIVE To explore the molecule recognition capability of valsartan-molecularly imprinted polymer(ValMIP) by HPLC. METHODS The chromatographic column of Val-MIP was synthesized by using valsartan as the template. Chromatographic performance of valsartan was investigated in different mobile phases. The Val-MIP was investigated for the recognition of valsartan and other compounds in the same mobile phase. RESULTS The Val-MIP exhibited high affinity for valsartan in the mobile phase of methanol and had good selectivity for valsartan and chlorogenic acid. The K' value reduced as the content of polar solvent increased in the mobile phase. The Val-MIP had no selectivity in the mobile phase of methanol-acetic acid(7∶3). CONCLUSION The Val-MIP has good selectivity for valsartan and chlorogenic acid acid. The Val-MIP can be used for extracting analogs of valsartan from herbs.
出处
《中国现代应用药学》
CAS
CSCD
2015年第10期1210-1214,共5页
Chinese Journal of Modern Applied Pharmacy
基金
广东省教育厅科技创新项目(2012KJCX0035)
广东省中医院中医药科学技术研究专项(YN2014ZH07)
关键词
缬沙坦
分子印迹聚合物
分子识别
绿原酸
valsartan
molecularly imprinted polymer
molecule recognition
chlorogenic acid