期刊文献+

外源ABA对苗期低温下冬小麦蔗糖含量及其关键酶基因表达的影响 被引量:5

Effect of Exogenous Abscisic Acid on Sucrose Content and Expression of Key Enzyme Genes Controlling Sucrose Metabolism in Seedling Period of Winter Wheat under Cold Stress
下载PDF
导出
摘要 为了探讨外源ABA对苗期低温下冬小麦植株蔗糖代谢的调节作用,以抗寒性强的品种东农冬麦1号和抗寒性弱的品种济麦22为试验材料,在三叶期分别于不同温度下经ABA处理48 h后取叶片和地下茎,研究外源ABA对苗期低温下冬小麦的蔗糖含量及蔗糖代谢相关酶基因表达的影响。结果表明,外源ABA处理使低温下抗寒性强的东农冬麦1号中积累了更多的蔗糖,尤其是叶片,而在济麦22中则抑制了蔗糖的积累。4℃以上温度时,外源ABA促进了东农冬麦1号叶片和地下茎中UGP在蔗糖合成中的作用;在4℃以下低温时,外源ABA则促进了UGP在蔗糖分解中的作用。4℃以上温度时,外源ABA提高了东农冬麦1号叶片中Ta SPS基因和地下茎中Ta SAInv基因的表达,而抑制了济麦22各器官中Ta SPS基因和Ta SAInv基因的表达。此外,4℃以上温度时,外源ABA抑制了两个小麦品种各器官中Ta SS基因的表达。表明抗寒性强的东农冬麦1号对外源ABA可能更加敏感,其蔗糖合成能力的提高将有利于冬小麦植株抵御低温,进而维持植株的存活。 To discuss the regulation of exogenous ABA on sucrose metabolism in seedling stage of winter wheat,two winter wheat cultivars Dongnongdongmai 1 with strong cold resistance and Jimai 22 with weak cold resistance were used as materials. The leaves were sprayed with ABA at trefoil stage. Leaves and underground stems were collected to study the effects of exogenous ABA on sucrose content and expression of key enzyme genes controlling sucrose metabolism. The results indicated that with the temperature decrease in seedling stage,Dongnongdongmai 1 accumulated more sucrose with exogenous ABA,especially in leaves,while the sucrose content was inhibited by exogenous ABA in Jimai 22. With the treatment of exogenous ABA,UGP played a major role in the synthesis of sucrose at the temperature above 4 ℃,and played a major role in the decomposition of sucrose at the temperature below 4 ℃. The expression of Ta SPS gene in leaves and Ta SAInv gene in underground stems of Dongnongdongmai 1 were promoted by exogenous ABA at the temperature above 4 ℃,but the expression of Ta SPS and Ta SAInv genes were inhibited by exogenous ABA in two organs of Jimai 22.The expression of Ta SS genes in different organs of two wheat varieties were inhibited by exogenous ABA. It showed that Dongnongdongmai 1 with strong cold resistance could be more sensitive to exogenous ABA. The ability of sucrose synthesis will help to resist the low temperature and maintain the plant survival.
出处 《麦类作物学报》 CAS CSCD 北大核心 2015年第10期1333-1340,共8页 Journal of Triticeae Crops
基金 黑龙江省自然科学基金资助项目(C2015047) 齐齐哈尔大学青年教师科研启动支持计划项目(2014k-M19) 国家自然科学基金项目(31471423) 东北农业大学博士启动基金项目(12RCB110)
关键词 冬小麦 ABA 苗期 蔗糖含量 蔗糖代谢酶基因 Winter wheat Abscisic acid Seedling period Sucrose content Sucrose metabolism enzyme genes
  • 相关文献

参考文献16

  • 1于晶,张林,崔红,张永侠,苍晶,郝再彬,李卓夫.高寒地区冬小麦东农冬麦1号越冬前的生理生化特性[J].作物学报,2008,34(11):2019-2025. 被引量:63
  • 2刘玉凤,李天来,焦晓赤.短期夜间亚低温及恢复对番茄光合作用和蔗糖代谢的影响[J].园艺学报,2011,38(4):683-691. 被引量:39
  • 3Klimov S V,Astakhova N V,Bocharova M A,et al. Low tem perature tolerance of photosynthesis and carbohydrate meta bolic patterns account for the difference in tomato and cucum bet cold tolerance [J]. Russian Journal of Plant Physiology 1996,43:783-789.
  • 4JiaQH(贾庆虎).Effect of ABA on coldresistance of cucum-ber seedlings[D].Tai’an:Shandong Agriculture University(泰安:山东农业大学),2007.
  • 5ZhangZX(张中霞),BaiLH(自立华),CaiGF(蔡贵芳).Effect of ABA on sucrose accumulation of mature hetao melonfruit[J].园艺学报,2011,38(supp1ement):2607.
  • 6Ildiko K,Eva B S,Gdbor G. Cold acclimation and abscisic acid induced alterations in carbohydrate content in calli of wheat genotypes differing in frost tolerance [J]. Journal of Plant Physiology, 2004,161 (3) : 131-133.
  • 7Wang S H,Hu L P,Sun J L,et al. Effects of exogenous ab- scisic acid on leaf carbohydrate metabolism during cucumber seedling dehydration [J]. Plant Growth Regulation, 2012,66 (1) :87-93.
  • 8高振宇,黄大年.植物中蔗糖和淀粉合成的关键酶 Ⅱ.尿苷二磷酸-葡萄糖焦磷酸化酶[J].生命的化学,1998,18(4):30-34. 被引量:5
  • 9张伟,张惠君,王海英,谢甫绨,陈振武.株行距和种植密度对高油大豆农艺性状及产量的影响[J].大豆科学,2006,25(3):283-287. 被引量:74
  • 10Worrell A C, Bruneau J M, Summerfelt K, et al. Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning [J]. Plant Cell, 1991, 3 : 1121-1130.

二级参考文献78

共引文献194

同被引文献111

引证文献5

二级引证文献26

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部