低温结晶法富集鳡鱼内脏油中多不饱和脂肪酸

Enrichment of polyunsaturated fatty acids from Yellow cheek carp( Elopichthys bambusa) viscera oil by low temperature crystallization

以95%乙醇为溶剂,碘值和回收率为指标,在单因素试验的基础上,运用正交试验优化低温结晶法富集鳡鱼内脏油中多不饱和脂肪酸(PUFA)的工艺条件。结果表明,最佳工艺条件为:料液比1∶6,结晶温度-30℃,结晶时间5 h;在最佳条件下,PUFA产品的碘值(I)为203.80 g/100 g,回收率为72.82%;富集后PUFA含量提高了24.26个百分点,其中,二十碳五烯酸(EPA)+二十二碳六烯酸(DHA)含量提高了3.81个百分点。

With 95% ethanol as solvent,iodine value and recovery rate as indicators,the process condi- tions of concentrating polyunsaturated fatty acids （PUFA） from Yellow cheek carp viscera oil by low tem- perature crystallization were optimized by orthogonal experiment based on the single factor experiment. The results showed that the optimal enrichment conditions were obtained as follows ： ratio of material to liq- uid 1 ： 6, crystallization temperature - 30 ℃ and crystallization time 5 h. Under these conditions, the iodine value and recovery rate of the PUFA product were 203：80 gI/100 g and 72.82% respectively. After en- richment, the PUFA content of Yellow cheek carp viscera oil increased by 24.26 percentage points, in which the total content of EPA and DHA increased by 3.81 percentage points.