不同碳氮源浓度和培养温度对裂殖壶菌产DHA的影响

Impacts of different concentrations of carbon and nitrogen sources and culture temperature on DHA production by Schizochytrium sp.

研究不同碳氮源浓度和培养温度对裂殖壶菌产DHA的影响。用干重法测定裂殖壶菌的生物量,溶剂法提取油脂,并用GC分析DHA含量。结果表明:最适初始葡萄糖质量浓度和氮源质量浓度分别为80、15 g/L;菌种在26℃下培养48 h后,再将发酵温度降到22℃下培养120 h,其DHA含量为43.62%,DHA产量为5.23 g/L。在最佳条件下,50 L放大培养(先26℃培养48 h,后22℃培养96 h)后油脂产量可达14.8 g/L,DHA产量可达6.5 g/L。综上所述,裂殖壶菌具有较好的工业化生产潜力。

The effects of different concentrations of carbon and nitrogen sources and culture temperature on DHA production by Schizochytrium sp. were studied. The biomass of Schizochytrium sp. was deter- mined by dry weight method, the oil was extracted by solvent method, and the content of DHA in the Schizochytrium sp. oil was analyzed by GC. The results showed that the optimal mass concentrations of in- itial glucose and nitrogen source were 80 g/L and 15 g/L, respectively; when the strain was first cultured at 26℃ for 48 h ,then cultured at 22 ℃ for 120 h,the content and yield of DHA in the Schizochytrium sp. oil were 43.62% and 5.23 g/L,respectively; the 50 L pilot - scale fermentation experiment （first cultu- ring at 26 ℃for 48 h, then culturing at 22 ℃ for 96 h） was conducted under the optimal conditions, and the yields of Schizochytrium sp. oil and DHA reached 14.8 g/L and 6.5 g/L respectively. In summary, Schizochytrium sp. had good industrial production potential.