摘要
流式微球技术是一种仅需少量样品即可实现多种待测目标成分实时、快速检测的新型分析技术,具有高通量、高特异性、高灵敏性、操作简便、低成本、重复性好、快速检测等优势,已被广泛用于食品、农产品、环境等领域内各种目标成分的检测研究,近年来尤其在小分子物质检测方面取得较快进展。该文介绍了流式微球技术的原理,总结了其在真菌毒素、农药残留、贝类毒素等小分子物质检测中的应用,并展望了流式微球技术在中药复杂基质中痕量小分子物质检测的应用及其发展趋势。
Cytometric bead array( CBA) is a new analytical technique,which can achieve real-time and rapid detection of targeted components in a small amount of sample. With many advantages of high throughput screening,high specificity and sensitivity,low cost,easy operation and good repeatability,this CBA technique has been widely used for the detection of various components in foods,agricultural products and environmental samples. Recently,it has got significant development in rapid detection of small molecules.This review briefly introduced the theory of CBA technique,summarized the application in the analysis of small molecules,such as mycotoxins,pesticide residues,shellfish toxins,and then prospected the application of trace small molecules detection in the complex matrices of traditional Chinese medicine and the development trend of it.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第18期3515-3523,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81473346
81274072)
协和新星人才支持计划项目
关键词
流式微球技术
小分子物质
快速检测
高通量
应用进展
cytometric bead array
small molecules
rapid detection
high throughput
application progress