姜辣素拮抗半夏凝集素蛋白刺激巨噬细胞所致炎症因子TNF-α释放增加、ROS过量生成及RIP3表达增高

Antagonistic effect of gingerols against TNF-α release,ROS overproduction and RIP3 expression increase induced by lectin from Pinellia ternata

为探索姜辣素拮抗半夏凝集素蛋白致炎作用,该研究采用ELISA法测定生姜不同提取部位对半夏凝集素蛋白所致巨噬细胞释放炎症因子TNF-α的影响;采用荧光探针测定姜辣素对半夏凝集素蛋白刺激巨噬细胞氧自由基ROS水平变化的影响;采用Western blot法研究姜辣素对半夏凝集素蛋白致巨噬细胞中细胞受体相互作用蛋白RIP3的表达水平增高的影响;扫描电镜研究姜辣素对半夏凝集素蛋白致巨噬细胞形态变化的影响。结果显示姜辣素可显著抑制半夏凝集素蛋白刺激巨噬细胞所导致炎症因子释放增加、ROS过量生成及RIP3的表达增高,扫描电镜显示姜辣素可抑制半夏凝集素蛋白导致的细胞变形坏死。生姜解半夏毒的机制可能与姜辣素抑制半夏凝集素蛋白刺激巨噬细胞导致的炎症因子释放、ROS的过量生成、RIP3的表达增高及巨噬细胞坏死相关,机体表现为抑制炎症的发生发展。

To explore the antagonistic effect of gingerols against the inflammation induced by lectin from Pinellia ternata. In this study,ELISA method was used to determine the effect of different extracts from gingerols on the release of inflammatory factor TNF-αfrom macrophages induced by lectin from P. ternata. The fluorescence probe was used to determine the effect of gingerols on the changes in ROS of macrophages induced by lectin from P. ternata. The western-blot method was applied to study the effect of gingerols on the increase in expression of cell receptor interacting protein RIP3 in macrophages induced by lectin from P. ternata. The scanning electron microscope（ SEM） was used to study the effect of gingerols on morphological changes in macrophages induced by lectin from P. ternata. According to the results,gingerols can significantly inhibit the release of inflammatory factor from macrophages induced by lectin from P. ternata,ROS overproduction and increase in RIP3 expression. SEM results showed that gingerols can inhibit the cytomorphosis and necrocytosis induced by lectin from P. ternata. Fresh ginger＇s detoxication may be related to gingerols＇ effects in inhibiting release of inflammatory factor,ROS overproduction and increase in RIP3 expression caused by macrophages induced by lectin from P. ternata,which are mainly inflammatory development.