鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究

Polymorphism in PLIN Gene Intron 6 and its Association with Carcass and Fatness Traits in Chicken

试验采用PCR-RFLP方法检测脂滴包被蛋白(perilipin,PLIN)基因内含子6在济宁百日鸡、莱芜黑鸡等4个地方鸡种和1个培育品系中的遗传多态性,分析了多态位点不同基因型与鸡胴体及脂肪性状的相关性。结果发现,在5个供试群体中检测到1个多态位点,测序证实为新发现的鸡PLIN基因2 467bp处G→A突变,该突变位点在供试群体中检测到3种基因型:A1A1、A1A2和A2A2,2个等位基因:A1和A2。等位基因A1在所有供试群体中均表现为优势等位基因。关联分析结果表明,PLIN基因2 467bp位点对鸡部分胴体性状和脂肪性状影响显著(P<0.05)。多重比较结果表明,A1A1基因型个体活体重、屠体重、全净膛重、腹脂重和腹脂率均显著高于A2A2基因型个体(P<0.05)。A1A2基因型个体的胸肌肌内脂肪含量显著高于A1A1和A2A2基因型个体(P<0.05)。研究结果表明,PLIN基因对鸡胴体及脂肪性状有一定影响。

The genetic polymorphisms in PLINgene were detected by PCR-RFLP method in four Chinese local chickens including Jining Bairi chicken,Laiwu Black chicken et al.,and one breeding strain.The correlations between the SNP and the carcass and fatness traits were analyzed.As a result,a novel G→A mutation at 2 467 bp in PLIN gene was identified in the five populations.Three genotypes（A1A1,A1A2 and A2A2）were detected,and allele A1 was predominant in all the five experimental populations.The statistical analysis showed that the 2 467 bp polymorphism locus was significant association with some carcass and fatness traits（P〈0.05）.The living body weight,carcass weight,eviscerated weight,abdominal fat weight and percentage of abdominal fat of A1A1 genotype was higher than A2A2 genotype in chickens（P〈0.05）.The breast intramuscular fat of A1A2 genotype was higher than that of A1A1 and A2A2genotypes in chickens（P〈0.05）.The results showed that PLIN gene had effect on carcass and fatness traits in chickens.