摘要
为了建立那西肽预混剂组分测定的高效液相检查法,采用十八烷基键合硅胶为填充剂,以0.025%磷酸水溶液-乙腈(50:50)为流动相,检测波长为241 nm,并以该色谱条件对来自7个厂家的14批不同规格的那西肽预混剂进行了测定。综合结果给出组分的建议限度为:那西肽组分A的峰面积不得少于那西肽组分A与组分B峰面积之和的88.0%。该方法具有专属性强、耐用性好、操作简便等优点。
The HPLC method of component determination for Nosiheptide premix was established. The operation conditions were performed on C18 column with a mobile phase of 0. 025% phosphoric acid solution -acetonitrile （50：50）. The detection wavelength was 241 nm. According to this method, the component of 14 batches of Nosiheptide premix from 7 manufacturers was determined. Based on the results, a proposed limitation of the component was given： the peak area of Nosiheptide component A can not less than 88.0% of the total peak area of component A and B. This method has special property, good durability and simple operation.
出处
《中国兽药杂志》
北大核心
2015年第10期27-31,共5页
Chinese Journal of Veterinary Drug
