淋病奈瑟球菌对头孢菌素类药物的敏感性及多抗原序列分型研究

Analysis on sensitivity of Neisseria gonorrhoea to cephalosporin antibiotics and multi-antigen sequence typing

目的了解广州市临床分离淋病奈瑟球菌（NG）的分子型别及其对头孢菌素类抗生素的敏感性。方法收集广东省皮肤性病防治中心临床分离的121株NG，用琼脂稀释法测定菌株对头孢曲松和头孢克肟的最小抑菌浓度（MIC），并进行NG多抗原测序分型（NG—MAST）。结果未发现NG对头孢曲松和头孢克肟耐药的菌株，但它们的低敏率分别达到13．2％（16／121）和2．5％（3／121）。121株NG共有82个型别，其中STl768共6株，ST2083、ST5062和ST10229各4株，ST1766、ST1866和ST10339各3株，有12个ST族群包含2株菌株，其余63个型别仅含1株菌株，占76．8％（63／121）。对头孢曲松低敏的13株菌株中，ST10339和ST10242各2株，其余9株均为单一型别。结论121株NG对头孢曲松、头孢克肟的敏感性较高。菌株NG—MAST型别具有较高的多样性。

Objective To understand the molecular types of clinical isolated Neisseria gonorrhoeae （NG） in Guangzhou, and to analyze the sensitivity to the cephalosporin antibiotics. Methods A total of 121 NG strains isolated from Guangzhou Skin Disease Prevention and Control Center were included in this study. The agar dilution method was used to determine the minimum inhibitory concentrations （MIC） to ceftriaxone and cefixime. DNA was extracted for NG multi-antigen sequence typing （NG-MAST）. Results The drug-resistance analysis showed that none of the strains appeared to be resistant to eeftriaxone and eefixime. However, the low sensitivity rates were 13.2% （16/121 ） and 2.5% （3/121）, respectively. A total of 82 genotypes were identified among the 121 isolates. The most prevalent genotype was ST1768 （n=6） in NG strains isolated, followed by ST2083 （n=4）, ST5062 （n=4）, ST10229 （n=4）, ST1766 （n=3）, ST1866 （n=3）, and ST10339 （n=3）. There were 12 genotypes containing 2 NG strains, and 63 genotypes [76.8% （631121） ] containing only one NG strain. Among the 13 NG strains with low sensitivity to eeftriaxone, there were 2 strains of ST10339 and 2 strains of ST10242, and the rest were single genotype. Conclusions The 121 NG strains have relatively high sensitivity to eeftriaxone and eefixime. The genotypes of these NG-MAST strains are high diversity.