摘要
目的:在建立小型猪下颌骨放射性骨坏死动物模型基础上,应用电镜技术研究下颌骨放射性骨坏死形成、发展的超微结构动态变化过程。方法:对6只小型猪右侧下颌骨进行25 Gy和28 Gy一次性照射,建立小型猪右侧下颌骨放射性骨坏死动物模型,分别于照射结束后3、4、5个月切取右侧下颌骨放射性骨坏死部位死骨,制备标本,进行电镜检查分析。结果:照射后的早期骨胶原纤维即受到破坏,骨细胞先出现细胞膜破坏,随后胞质出现空泡,细胞器裂解,最后出现核变化。大剂量照射后骨细胞的破坏出现早而重,并且加速了骨细胞的裂解、死亡。结论:骨细胞细胞膜及细胞质较细胞核对放射线更为敏感,照射后骨细胞死亡过程首先发生在细胞膜及细胞质内,与以往认为细胞核损伤在先的观点不同。
PURPOSE： After establishing the animal model, electron microscope was used to observe the dynamic ultrastructural changes during the process of mandibular osteoradionecrosis（ORN） for investigation of the possible pathogenesis. METHODS： A single dose of irradiation（IR25Gy, 28Gy） was delivered via a electronic liner accelerator to right mandible of 6 miniature pigs by utilizing the 3D-CRT technique. Samples of sequestrations were extracted at 3, 4and 5 months after irradiation for electron microscopic analysis. RESULTS： Osteocollagenous fibers were destroyed shortly after irradiation. Cellular membrane was destroyed at early stage. Then vacuole formed in the cytoplasm and organelle began to split. At last, nucleus disaggregated. CONCLUSIONS： Doses of irradiation are closely related to degree of sequestration. Cellular membrane and cytoplasm are more sensitive to the irradiation than cellular nucleus which is different from the previous findings.
出处
《中国口腔颌面外科杂志》
CAS
2015年第5期395-399,共5页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(81170935)~~
关键词
放射性骨坏死
小型猪
电镜
Osteoradionecrosis
Miniature pigs
Electron microscope
