脓毒症大鼠急性肺损伤机制研究

Mechanism of Acute Pulmonary Injury in Rats with Sepsis

目的探讨脓毒症大鼠急性肺损伤的机制。方法采用盲肠结扎穿孔术(CLP)建立大鼠脓毒症模型。健康成年雄性SD大鼠48只,随机分为假手术组、脓毒症组,每组24只。动物模型制备成功后,于术后3、12、24h检测肺组织匀浆标本黄嘌呤氧化酶(XO)、丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量。光学显微镜下观察肺组织病理改变。结果术后3、12、24h假手术组XO分别为(2.4±0.3)U/g、(2.3±0.5)U/g、(2.4±0.4)U/g,MDA为(15.7±2.7)nmol/mg、(15.2±2.9)nmol/mg、(15.6±2.6)nmol/mg,SOD为(58.8±8.0)U/mg、(58.1±10.1)U/mg、(58.7±9.5)U/mg,GSH-Px为(33.2±5.4)U/mg、(32.5±7.0)U/mg、(33.1±6.3)U/mg;脓毒症组XO分别为(4.2±0.6)U/g、(5.5±0.7)U/g、(5.7±0.5)U/g,MDA为(29.3±5.7)nmol/mg、(36.3±6.6)nmol/mg、(41.4±7.3)nmol/mg,SOD为(43.3±7.0)U/mg、(41.8±6.9)U/mg、(38.2±7.4)U/mg,GSH-Px为(27.9±4.0)U/mg、(26.4±3.9)U/mg、(24.3±3.3)U/mg。与假手术组比较,脓毒症大鼠肺组织XO、MDA含量显著升高,SOD、GSH-Px含量显著降低,差异有统计学意义(P<0.01)。结论脓毒症大鼠存在急性肺损伤,其机制可能与氧化应激相关。

Objective To investigate the mechanism of acute pulmonary injury in sepsis rats. Methods Colon ligation perforation was applied to rats to establish sepsis model. Forty-eight healthy adult male SD rats were ran-domly divided into sham group and sepsis group（n=24 in each group）. At 3, 12, 24h after surgery, every 8 rats in each group were sacrificed to obtain lung tissue for determination of xanthine oxidase（XO）, malonaldehyde（MDA）, superoxide dismutase（SOD）, and glutathione peroxidase（GSH-Px）. Pathological changes of lung tissue was observed with light microscopy. Results At 3, 12, 24h after surgery in sham group, XO were 2.4±0.3U/g, 2.3±0.5U/g, and 2.4±0.4U/g, MDA were 15.7±2.7nmol/mg, 15.2±2.9nmol/mg, 15.6±2.6nmol/mg, SOD were 58.8±8.0U/mg, 58.1±10.1U/mg, 58.7±9.5U/mg, and GSH-Px were 33.2±5.4U/mg, 32.5±7.0U/mg, 33.1±6.3U/mg; in sepsis group, XO were 4.2±0.6U/g, 5.5±0.7U/g, and 5.7±0.5U/g, MDA were 29.3±5.7nmol/mg, 36.3±6.6nmol/mg, 41.4±7.3nmol/mg, SOD were 43.3±7.0U/mg, 41.8±6.9U/mg, 38.2±7.4U/mg, and GSH-Px were 27.9±4.0U/mg, 26.4±3.9U/mg, 24.3±3.3U/mg. Com-pared with sham group, the concentration of XO and MDA were significantly increased and SOD and GSH-Px were significantly decreased in sepsis group（P〈0.01）. Conclusion Sepsis rats suffer from acute pulmonary injury, which may be related to oxidative stress.