柚子皮渣生产单细胞蛋白饲料工艺研究

Study on the Process of Producing Single Cell Protein Feed by the Skin of Grapefruit

以柚子皮渣为原料,以康宁木霉、白地霉、酵母菌、产朊假丝酵母为菌体,用卵清蛋白为标准品和双缩脲试剂制定标准曲线,探究单菌株与混合菌株分别固态发酵后产单细胞蛋白的含量。结果表明:白地霉与康宁木霉组合菌发酵效果最好,培养基发酵7d后蛋白质含量达到8.060g,占培养基干重的19%左右。发酵效果受温度、p H值、接种量、培养基含水量等因素影响,用正交实验获得白地霉与康宁木霉最佳发酵条件组合为:最适p H值5.5,最适发酵温度25℃,最适接种量15%,最适培养基含水量65%,且各因素对发酵的影响大小为发酵温度>培养基含水量>接种量>p H。

Taking grapefruit bran as raw material,koningii,white mold,yeast,geotrichum as fermentation bacteria. through the standard curve with ovalbumin and Biuret reagent. the paper probed the single cell protein content after solid-state fermentation of single strain and mixed strain. It was found that the combination of Geotrichum with kon？ingii has the best fermentation effect,and after 7d fermentation the medium content 8.060g protein,accounting for about 19%of the dry weight of the medium.Fermentation affected by the temperature,pH,inoculum size,medium’s water content. The orthogonal experiment showed that the combination of Geotrichum with koningii ’s best fermenta？tion condition ,the optimum pH value is 5.5,the optimum fermentation temperature is 25℃,the optimum inoculation amount is 15%,the optimum medium moisture content is 65%. The impact of various factors on fermentation is fer？mentation temperature〉medium’s water content〉inoculation amount〉pH.