祛浊通痹方抗炎镇痛作用及对脂多糖诱导Raw264.7细胞的抗炎活性

Anti-inflammatory and analgesic effect of Quzhuo Tongbi Formula on Raw264.7 cells induced by LPS

目的:研究祛浊通痹方(QZTB)抗炎镇痛及对脂多糖(LPS)诱导Raw264.7细胞的抗炎活性。方法:采用二甲苯致小鼠耳肿胀法评价QZTB抗炎作用以及热板法和醋酸致扭体法评价其镇痛作用。ELISA法测定LPS诱导Raw264.7细胞培养上清的前列腺素E2(PGE2)含量。结果:QZTB中、高剂量组能有效缓解小鼠二甲苯所致的耳廓肿胀(P<0.05,P<0.01),而QZTB低、中、高剂量组均明显提高小鼠痛阈值,并显著减少醋酸致小鼠扭体次数(P<0.01)。QZTB对LPS诱导Raw264.7细胞中PGE2的产生具有明显抑制作用(P<0.05,P<0.01)。结论:QZTB具有较好的抗炎镇痛作用及对体外LPS诱导Raw264.7炎性细胞具有抗炎活性,为进一步研究QZTB治疗痛风作用机制提供一定的理论依据。

Objective：To study the anti-inflammatory and analgesic effects of Quzhuo Tongbi Formula（QZTB） and its anti-inflammatory activities in Raw264.7 cells induced by lipopolysaccharide（LPS）.Methods：Mouse models of ear swelling induced by dimethylbenzene were used to evaluate the anti-inflammatory action,and hot plate test and acetic acid twisting method were used to evaluate the analgesic effect.The concentration of PGE_2 in culture supernatants of Raw264.7 cells induced by LPS was tested by using ELISA.Results：QZTB could relieve the ear swelling of mouse induced by dimethylbenzene（P〈0.05,P〈0.01）,improve the pain threshold of mouse,reduce the torsion times of mouse induced byacetic acid（P〈0.01）,and inhibit the generation of PGE_2 in culture supernatants of Raw264.7 cells induced by LPS obviously（P〈0.05,P〈0.01）.Conclusion：QZTB has obvious anti-inflammatory and analgesic effects and shows ananti-inflammatory activity in LPS-induced Raw264.7 cells in vitro,which provids a scientific theoretical basis for the further studies about the mechanism of treating gout with QZTB.