癃畅颗粒对人前列腺基质细胞增生及凋亡的影响

Effect of Longchang Granule on Proliferation and Apoptosis of Prostate Stromal Cells

目的:探讨癃畅颗粒对体外培养的人前列腺基质细胞增生及凋亡的影响。方法:原代培养增生的人前列腺基质细胞,分别以高、中、低浓度癃畅颗粒处理,MTT法测定细胞增殖指数,TUNEL法检测细胞凋亡。结果:癃畅颗粒处理实验组24 h后前列腺基质细胞的生长活性均被不同程度的抑制,高、中、低浓度组抗增殖指数分别为(0.273±0.0881)%,(0.418±0.0644)%和(0.674±0.0352)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,细胞抗增殖指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05);药物处理后细胞凋亡百分率显著增加,高、中、低浓度组凋亡指数分别为(22.12±3.47)%,(44.96±2.81)%和(80.36±10.16)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,凋亡指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05)。结论:癃畅颗粒能够显著抑制体外培养的人前列腺基质细胞增殖,促进前列腺基质细胞凋亡。

ObjectiveTo investigate the effect of Longchang granules on the proliferation and apoptosis ofprostate stromal cells in vitro and to provide the theoretical basis for Longchang granules to treat benign prostat-ic hyperplasia.MethodsLongchang granules at low,medium and high doses were prepared and added to theprostate stromal cells in vitro.The proliferation of cells was tested by MTT.The apoptosis of cells was identifiedby TUNEL assay.ResultsAfter being treated for 24 h,there were statistical differences between blank groupand longchang groups in the proliferation of prostate stromal cells（P 〈0.05） and there were also significant dif-ferences between high-dose,medium-dose and low-dose groups（P 〈0.05）.The results tested by TUNEL assayshowed that there were statistical differences between blank group and longchang groups in the apoptosis of pros-tate stromal cells（P 〈0.05）,and there were also significant differences between high-dose,medium-dose andlow-dose groups（P 〈0.05）.ConclusionLongchang granules can significantly inhibit the proliferation andpromote the apoptosis of prostate stromal cells in vitro.