利多卡因后处理对家兔心肌缺血-再灌注损伤TNF-α NF-κB表达的影响

The effect of lidocaine post-treatment on TNF-αand NF-κB in rabbits with myocardial ischemia reperfusion injury

目的研究家兔心肌缺血-再灌注损伤时,利多卡因后处理对其体内TNF-α水平及NF-κB表达的影响。方法 18只家兔随机分为假手术组、缺血-再灌注组和利多卡因组3组。结扎家兔心脏的左室支40min后解除结扎,恢复血液供应,再灌注2h后测定各组家兔血浆TNF-α的水平,通过免疫组化染色和免疫印迹方法观察心肌NF-κB的表达情况。结果与缺血-再灌注组比较,利多卡因组再灌注后血浆TNF-α浓度明显降低,心肌细胞NF-κB的表达显著减少。结论利多卡因可以抑制再灌注心肌炎症反应,减轻损伤。

Objective To investigate the protective effects of lidocaine post-treatment on TNF-αand NF-κB in rabbits with myocardial ischemia reperfusion injury.Methods Eighteen rabbits were randomly divided into 3 groups,sham operation group,ischemic reperfusion group and lidocaine group.The left ventricular branch （LVB） of rabbits was ligated for 40minutes,then the ligation was removed.Afer 120 minutes the level of plasma TNF-αwas measured,meanwhile the degree of NF-κB was detected with immumohistochemical staining and Western blot. Results Compared with those of ischemic reperfusion group,the concentration of TNF-αwas decreased in lidocaine group （P 〈0.01）.The expression of NF-κB was inhibited by lidocaine too.Conclusion The results demonstrated that lidocaine could protect cardiac function,inhibit inflammatory response and attenuate reperfusion injury in is-chemic heart.