匙羹藤愈伤组织培养研究

Study on Callus Culture from Gymnema sylvestre

以匙羹藤组培苗为外植体,研究茎段、茎尖和叶片以及不同植物生长调节剂组合对愈伤组织形成的影响;采用4因素4水平正交设计,用SPSS软件其结果进行分析。结果显示:各种外植体诱导愈伤的能力不同,依次是茎段、叶片。叶片诱导出非胚性愈伤组织,茎段、茎尖利于诱导胚性愈伤组织和胚状体;愈伤诱导的最佳培养基为MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L,诱导率为84.5%,愈伤生长旺盛,分裂快,颜色为淡黄色。愈伤组织分化成苗效果最好的条件是MS+NAA 1.0 mg/L+IBA 0.1 mg/L,分化率达到90.6%,最佳的生根条件是1/2 MS+NAA1.6 mg/L,生根率为100%。这为研究匙羹藤的组织的脱分化、分化以及胚状体的形成机制提供了参考。

Using Gymnema sylvestre as explants to study how stems,stem tips,leaves and hormone affect callus. Using 4 factors and 4 levels orthogonal to design,using SPSS to analyze results. Results displayed that all kinds of explants have different callus induction abilities,followed by stems and leaves. Leaves inducedi non-embryogenic callus. Stems and stem tips induced embryogenic callus and embryoid. The best tissue culture of callus induction is MS ＋ 2,4-D 2. 0 mg / L ＋ 6-BA 0. 5 mg / L,the induction rate is 84. 5%,callus grown more vigorous,splinter grown faster,the color is faint yellow. The optimal medium for differentiation was MS ＋ IBA 0. 1 mg / L ＋ NAA 1. 0 mg / L. The inducing rate was 90. 6%,The optimal rooted medium was 1 /2 MS ＋ NAA 1. 6 mg / L. The rooting rate was 100%. This provides reference for dedifferentiation,differentiation,embryoid formation mechanism of Gymnema sylvestre organization.