高效液相色谱法测定碳酸钙D_3咀嚼片中维生素D_3的含量

Determination of Vitamin D_3 in Calcium Carbonate and Vitamin D_3 Chewable Tablets by HPLC

目的：建立高效液相色谱法测定碳酸钙D3咀嚼片中维生素D3含量的方法。方法：采用高效液相色谱法。以Diamonsil C18（250 mm×4.6mm,5μm）为色谱柱;流动相为甲醇;流速：1.0 ml·min-1;检测波长：264 nm。结果：维生素D3在4.8~38.4μg范围内。进样量与峰面积线性关系良好（r=1.0）。平均回收率为：97.82%（RSD=1.15%）。结论：方法简便、快速准确、灵敏度高,重现性好。可作为碳酸钙D3咀嚼片的质量控制方法。

Objective： To establish a method for the determination of vitamin D3 in Calcium Carbonate and Vitamin D3 Chewable Tablets by HPLC. Methods： The HPLC system consisted of Diamonsil C18column（ 250 mm × 4. 6 mm,5 μm）,acetonitrile-water（ 23 ∶ 77）; flow rate at 1. 0ml·min-1and detected with UV at 264 nm. Results： The method had good linear relationship within the rage 4. 8 ~ 38. 4 μg of vitamin D3（ r = 1. 0）. The result（ n = 6） of the recovery was 97. 82%（ RSD = 1. 15%. Conclusion： The method is simple,fast,rapid,accurate and suitable for the determination of preparation.