不同方法制备的党参复方多糖的抗氧化活性研究

Study on antioxidant activity of polysaccharides from compound codonopsis pilosula produced by different methods

目的:对不同方法制备的党参复方多糖的抗氧化活性进行研究,并对多糖纯化程度与其活性的相关性进行探索。方法:采用膜分离工艺制备党参复方总多糖,并对得到的总多糖进行脱蛋白、脱色等不同方法的纯化,测定各多糖产物的还原能力、清除羟基自由基、DPPH·自由基及超氧阴离子自由基的能力。结果:不同方法制备的三种党参复方多糖样品均呈现体外抗氧化活性,并且具有良好的量效关系;总多糖经脱蛋白、脱色等纯化步骤后体外抗氧化活性减弱。结论:该党参复方总多糖具有较好的体外抗氧化活性,制备活性多糖时,不应盲目脱蛋白、脱色。

Objective： To study the antioxidant activities of Codonopsis pilosula compound polysaccharides prepared by different methods, and explore the relationship between polysaccharide purification and its activity. Methods： Codonopsis pilosula compound polysaccharides were isolated by membrane separation, purified further by removing the protein and pigment. The antioxidant activities of all polysaccharide products were investigated using various assays in vitro, including reducing power and scavenging ability on hydroxyl radicals, DPPH radicals, superoxide radicals. Results： All the polysaccharides prepared by different methods showed antioxidant activity in vitro and good dose-effect relationships. The antioxidant activity of total polysaccharides became weaker after purifing by deproteinization and decoloration. Conclusion： Codonopsis pilosula compound polysaccharides had better antioxidant activity in vitro. When preparing active polysaccharide, proteins and pigments shouldn＇ t be removed blindly.