人WJ-MHCs移植对心肌梗死后心力衰竭大鼠TNF-α及NT-proBNP的影响

Effect of human umbilical cord WJ-MHCs on TNF-αand NT-proBNP in the rats with heart failure of acute myocardial infarction

目的观察人华通胶间充质干细胞(WJ-MHCs)对心肌梗死后心力衰竭大鼠肿瘤坏死因子α(TNF-α)及N末端B型脑利钠肽前体(NT-proBNP)的影响。方法采用80只雄性SD大鼠通过异丙肾上腺素多点皮下注射,剂量为200mg/kg,隔24h注射1次共2次。待大鼠存活1周建立模型后各取12只随机均衡分入WJ-MHCs移植组、普通对照组、空白对照组各12只健康大鼠,3组再分为移植前和移植后4周两个亚组。WJ-MHCs移植组于心肌梗死后1周移植以DAPI标记的WJ-MHCs,空白对照组及普通对照组不做处理正常饲养。分别于移植前和移植后4周检测大鼠心脏组织中TNF-α水平,移植后4周WJ-MHCs细胞在大鼠心脏中的情况,大鼠TNF-α和NT-proBNP的水平,左室射血分数(LVEF)。结果移植后WJ-MHCs移植组较移植前LVEF明显提高(P<0.05),较移植前及普通对照组血清TNF-α及NT-proBNP明显降低(P<0.05);移植后WJ-MHCs移植组较普通对照组心脏组织中TNF-α表达明显减少;WJ-MHCs移植组病死率(16.67%)较普通对照组病死率(33.33%)明显降低;免疫荧光显示移植后4周仍可检测到移植的WJ-MHCs。结论移植人WJ-MHCs可明显降低心梗后心衰大鼠心脏组织及循环中的TNF-α,降低循环中的NT-proBNP,提高心功能。

Objective To observe the influence of human umbilical cord wharton′s jelly-mesenchymal stem cells（WJ-MHCs）on the tumor necrosis factorα（TNF-α）and N-terminal pro-brain natriuretic peptide（NT-proBNP）in rats with heart failure of acute myocardial infarction.Methods Totally 80 male rat models of heart failure of acute myocardial infarction were made by isoprenaline（ISO）200mg/kg injected subcutaneously twice at an interval of 24 hours.After one week,24 survival rats were randomly divided into WJ-MHCs transplantation group and normal control group.Sham group was made of 12 health rats,and then each of the three groups was subdivided into pre-transplantation group and post-transplantation group 4weeks later.WJ-MHCs transplantation group was transplanted with WJ-MHCs with DAPI labeled after ISO injected one week.Sham group and normal group were untreated and normally bred.The left ventricular ejection fraction（LVEF）measured by before transplantation and post-transplantation4 weeks later.The injected cells and the expression of TNF-αwas measured.Results Compared to pre-transplantation group,WJMHCs transplantation group increased the LVEF（P〈0.05）;compared to pre-transplantation and normal control,WJ-MHCs transplantation group reduced the TNF-αand NT-proBNP in the serum（P〈0.05）and the expression of TNF-αfrom the heart tissue（P〈0.05）;compared to normal transplantation,WJ-MHCs transplantation group reduced the mortality from 33.3% to 16.7%;immunofluorescence demonstrated that transplanted cells were still found alive in the heart after transplantation 4weeks later.Conclusion Transplantation of WJ-MHCS down-regulates TNF-αand NT-proBNP in the serum in the serum and the expression of TNF-αfrom the heart tissue and up-regulates the LVEF in rats with heart failure of acute myocardial infarction.