白血病细胞中Beta-Arrestin1与EZH2分子结合研究

Analysis of the interaction of Beta-Arrestin1 and EZH2 in leukemia cells

目的:检测急性淋巴细胞白血病细胞(acute lymphoblastic leukemia,ALL)CCRF-CEM和Raji细胞中Beta-抑制蛋白1(Beta-Arrestin1)与Zeste增强子同源物-2蛋白(enhancer of Zeste homolog 2,EZH2)是否存在结合。方法:白血病细胞中,Western blot检测Beta-Arrestin1与EZH2表达水平,激光共聚焦(Confocal)检测Beta-Arrestin1与EZH2在细胞中的位置与共定位;免疫共沉淀(CO-IP)检测Beta-Arrestin1与EZH2结合能力。结果:Beta-Arrestin1与EZH2在白血病K562、CCRF-CEM和Raji细胞中均有表达。激光共聚焦结果显示Beta-Arrestin1与EZH2均在K562、CCRF-CEM和Raji细胞内共定位,CO-IP结果显示在K562,CCRF-CEM和Raji细胞中Beta-Arrestin1与EZH2结合。结论:在CCRF-CEM和Raji中,Beta-Arrestin1可与EZH2结合。

Objective：To test that if Beta-Arrestinl could bind with enhancer of Zeste homolog 2（EZH2） in acute lymphoblastic leukemia（ALL） CCRF-CEM and Raji cells. Methods：The protein levels of Beta-Arrestinl and EZH2 in the leukemia ceils were determined by Western blot. The location of Beta-Arrestinl and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation（CO-IP） was examined for the binding of Beta-Arrestinl with EZH2 in leukemia cells. Results：Western blot results showed that b/Beta-Arrestinl and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza- tion of Beta-Arrestinl and EZH2 in K562, CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestinl bind with EZH2 in three leukemia cells. Conclusion：Beta-Arrestin 1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.