摘要
目的:检测急性淋巴细胞白血病细胞(acute lymphoblastic leukemia,ALL)CCRF-CEM和Raji细胞中Beta-抑制蛋白1(Beta-Arrestin1)与Zeste增强子同源物-2蛋白(enhancer of Zeste homolog 2,EZH2)是否存在结合。方法:白血病细胞中,Western blot检测Beta-Arrestin1与EZH2表达水平,激光共聚焦(Confocal)检测Beta-Arrestin1与EZH2在细胞中的位置与共定位;免疫共沉淀(CO-IP)检测Beta-Arrestin1与EZH2结合能力。结果:Beta-Arrestin1与EZH2在白血病K562、CCRF-CEM和Raji细胞中均有表达。激光共聚焦结果显示Beta-Arrestin1与EZH2均在K562、CCRF-CEM和Raji细胞内共定位,CO-IP结果显示在K562,CCRF-CEM和Raji细胞中Beta-Arrestin1与EZH2结合。结论:在CCRF-CEM和Raji中,Beta-Arrestin1可与EZH2结合。
Objective:To test that if Beta-Arrestinl could bind with enhancer of Zeste homolog 2(EZH2) in acute lymphoblastic leukemia(ALL) CCRF-CEM and Raji cells. Methods:The protein levels of Beta-Arrestinl and EZH2 in the leukemia ceils were determined by Western blot. The location of Beta-Arrestinl and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation(CO-IP) was examined for the binding of Beta-Arrestinl with EZH2 in leukemia cells. Results:Western blot results showed that b/Beta-Arrestinl and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza- tion of Beta-Arrestinl and EZH2 in K562, CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestinl bind with EZH2 in three leukemia cells. Conclusion:Beta-Arrestin 1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2015年第9期1215-1218,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金面上资助项目(编号:81373444)