苯醚甲环唑降解菌BMJHZ-01的分离鉴定及降解影响因素

Isolation,identification and degradative characters of difenoconazole-degrading bacteria BMJHZ-01

从江苏某农药厂污水区污泥中分离筛选出一株对苯醚甲环唑降解能力较强的细菌BM JHZ-01,对其进行形态学观察、生理生化鉴定、16S r DNA序列同源性比对及系统发育树分析,初步确定其为硝基还原假单胞菌Pseudomonas nitroreducens。通过研究该菌株降解苯醚甲环唑的影响因素,得出其最适宜降解条件为:培养箱转速200 r/min,培养温度30℃,初始p H值为7.0。在此培养条件下,采用高效液相色谱法检测,发现菌株BMJHZ-01在5 d内对180 mg/L苯醚甲环唑的降解率可达90%以上。BMJHZ-01可以以最高质量浓度为180 mg/L的苯醚甲环唑为惟一碳源进行生长繁殖并将其降解,对不良环境的高度适应性使其在环境治理中具有明显优势。

Bacteria BMJHZ-01, a strain capable of degrading difenoconazole was isolated from sewage sludges at a pesticide factory district in Jiangsu province. The morphology, physiological and biochemical identification for this strain was studied. 16S rDNA sequence homology analysis and phylogenetic tree alignment system indicated it as the Pseudomonas nitroreducens. Based on the study of the influence factors of degradation of difenoconazole, the most suitable degradation conditions for BMJHZ-01 was obtained as： Incubator speed 200 r/min and culture temperature of 30 ℃, initial pH value of 7.0. High performance liquid chromatography were used to detect pesticide concentration and its degradation rate was calculated. Difenoconazole with concentration of 180 mg/L could degraded more than 90% by the bacteria BMJHZ-01 within five days. BMJHZ-01 could grow and propagate in the highest difenoconazole concentration of 180 mg/L. The research results imply that this strain may be ultilized in future environmental management of difenoconazole pollution.