钾离子通道阻滞剂4-氨基吡啶对人舌鳞癌细胞迁移和侵袭的影响

Effect of 4-AP on migration and invasion of human oral tongue squamous cell carcinoma cell line Tca8113 in vitro

目的:研究4-氨基吡啶(4-AP)对人舌鳞状细胞癌(OTSCC)Tca8113细胞体外迁徙和侵袭能力的影响。方法:采用划痕愈合方法及Transwell侵袭实验检测10、20、30 mmol/L 4-AP干预Tca8113细胞后对Tca8113细胞迁徙及侵袭能力的影响。结果:浓度分别为10 mmol/L、20 mmol/L、30 mmol/L 4-AP干预Tca8113细胞后,Tca8113细胞12 h迁徙率分别为(14.07±5.59)%、(11.34±1.79)%及(8.71±3.26)%;24 h的迁徙率分别为(22.20±5.30)%、(14.24±2.52)%及(11.65±2.97)%,均明显小于对照组(19.25±4.50)%和(33.16±6.40)%,差异具有显著性(P<0.01)。细胞侵袭实验结果显示,10 mmol/L、20 mmol/L、30 mmol/L的4-AP作用于Tca8113细胞24 h后穿膜细胞数分别为(112.20±24.36)、(28.87±6.47)、(11.80±5.77)个,随着4-AP浓度的升高,Tca8113穿膜细胞数明显下降,与对照组(179.73±16.14)相比,差异具有显著性(P<0.001)。结论:4-AP具有抑制Tca8113舌鳞癌细胞株体外迁徙侵袭的能力。

Objective：To explore the effect of 4-AP on the migration and invasion of Tca8113 cells, thus provide reference for clinical studies. Method： Wound healing assay and Transwell assay were employed to explore whether 4-AP （10, 20, 30 mmol / ID could inhibit the migration and invasion of Tca8113 cell. Result： ①The scratch healing assay obtained cell migration rates of （19.25±4.50） %, （14.07±5.59） %, （11.34±1.79） % and （8.71 ±3.26） % for Tca8113 cells treated with 10, 20, 30 mmol / L of 4-AP for 12 h while （33.16±6.40）%, （22.20±5.30）%, （14.24±2.52）% and （11.65±2.97）% for 24 h. The results showed that the migration rates were significantly difference between 4-AP goups （10, 20, 30 mmol/L）and control groups （P 〈0.01）.②The transwell invasion assay showed that with the increasing of 4-AP concentration, the Tca8113 cell number decrased. The number of cells permeating through the matrigel were （112.20±24.36）, （28.87±6.47）, （11.80±5.77） when the Tca8113 cells were treated with 10 mmol / L,20 mmol / L, 30 mmol / L 4-AP for 24 h,while the control group was （179.73±16.14）,and the difference was significant （F =392.045, P 〈0.001）. Conclusion：4-AP can significantly inhibited the migration and invasion of human tongue carcinoma cell line Tca8113.