线纹香茶菜中咖啡酸、新西兰牡荆苷2、异夏佛塔苷的含量动态变化研究

Study on the Content Dynamic Changes of Caffeic Acid,Vicenin-2 and Isoschaftoside in Rabdosia lophanthoides

目的：建立同时测定线纹香茶菜中咖啡酸、新西兰牡荆苷2、异夏佛塔苷含量的方法,以确定线纹香茶菜的最佳采摘期。方法：采用高效液相色谱法。色谱柱为Diamonsil C18,流动相为甲醇-0.1%磷酸溶液（梯度洗脱）,流速为0.8 ml/min,检测波长为334 nm,柱温为25℃,进样量为15μl。结果：咖啡酸、新西兰牡荆苷2、异夏佛塔苷检测的质量浓度线性范围分别为0.23～5.68、0.31～7.76、0.45～11.30μg/ml（r=0.999 9、0.999 8、0.999 9）;精密度、稳定性、重复性试验的RSD〈2.0%;加样回收率分别为95.1%～98.9%、97.4%～101.3%、95.5%～98.8%,RSD分别为1.8%、1.7%、1.4%（n=6）。采收于每年4-5月、7-8月的线纹香茶菜中上述3种成分含量最高。结论：该方法简便、准确、重复性好,可用于同时测定线纹香茶菜中咖啡酸、新西兰牡荆苷2、异夏佛塔苷的含量;线纹香茶菜的最佳采摘期为每年4-5月、7-8月。

OBJECTIVE： To establish a method for simultaneous determination of caffeic acid, vicenin-2 and isoschaftoside in Rabdosia lophanthoides to assess its optimal harvest period. METHODS： HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1% phosphoric acid solution （gradient elution） at a flow rate of 0.8 ml/min, detection wave- length was 334 nm, column temperature was 25℃ and volume injection was 15 μl. RESULTS ： The linear range was 0.23-5.68 μg/ml for caffeic acid（r=0.999 9）, 0.31-7.76 μg/ml for vicenin-2（r=0.999 8） and 0.45-11.30μg/ml for isoschafloside（r=0.999 9）; RSDs of precision, stability and reproducibility tests were lower than 2.0%, average recoveries were 95.1%-98.9% （RSD=1.8%, n=6）, 97.4% -101.3% （RSD= 1.7%, n=6） and 95.5% -98.8% （RSD= 1.4%, n=6）, respectively. The contents of above-men- tioned 3 ingredients of R. lophanthoides were the highest harvested in Apr. to May and Jul. to Aug. in a year. CONCLUSIONS： The method is simple, accurate and reproducible, and can be used for the simultaneous determination of caffeic acid, vicenin-2 and isoschaftoside in R. lophanthoides. The optimal harvest period is Apr. to May and Jul. to Aug. in a year.