铜绿假单胞菌抗菌色素的结构鉴定及稳定性检测

Structural characterization and stability of antibacterial pigment from Pseudomonas aeruginosa

铜绿假单胞菌（Pseudomonas aeruginosa）产生的拮抗物质对撑×绿杂交竹梢枯病病原菌具有明显的抑制作用。铜绿假单胞菌发酵液预处理后,采用三氯甲烷萃取得到活性粗提物,后经硅胶柱色谱分离及紫外洗脱检测,得到抑菌活性最强的黄色油状活性物质。经核磁共振和红外光谱检测,得出该活性物质是一种有芳香环的吩嗪类化合物,化学式为C14H10O2N2。色素稳定性的研究表明,光照、温度对活性色素的稳定性影响甚微;低浓度的氧化剂和还原剂对活性色素的稳定性影响较小,高浓度对其影响较大;色素在偏中性或碱性条件下（p H7~8）活性最大,当p H≤3时,色素性质不稳定,抑菌活性几近消失;金属离子对色素稳定性有不同程度的影响,其中Na＋、K＋、Ba2＋、Ca2＋、Ag＋对色素的影响较小,Mg2＋、Zn2＋、Ni2＋、EDTA-Na2对色素稳定性有较大的影响,而Al3＋、Cu2＋、Fe3＋、Pb2＋对色素活性影响很大,色素几乎失去活性。

The antagonistic substance of Pseudomonas aeruginosa can effectively antagonize Arthrinium phaeospermum（ Corda） M. B. Elli. The yellow oily substances with strong microbe- inhibiting activity were included through silica gel column chromatography and UV elution after pretreatment of the filtrate of fermentation broth adopting trichloromethane extraction. By nuclear magnetic resonance and infrared spectrum detection,the compound was proved a phenazine pigment with aromatic rings,and with the chemical formula C14H10O2N2. Furthermore,the pigment stability test showed that illumination and temperature exerted slight effects on pigment activity. Light concentrate oxidizer and reducing agent exerted small effects on it,while the heavy concentrate was on contrary. The pigment activity was up to its max when p H was 7- 8; when p H≤3,it was instability and almost with no bacteriostatic activity. Metallic ion could affect the pigment stability in varying degrees,Na＋,K＋,Ba2 ＋,Ca2 ＋and Ag＋exerted a light effect on pigment＇s stability,however,Al3 ＋,Cu2 ＋,Fe3 ＋,Pb2 ＋exerted a heavy influence,which nearly caused it lost all activities.