miR-1284过表达对人胃癌SGC-7901细胞基因表达谱及侵袭转移的影响

The effect of mi R-1284 over-expression on gene expression profiling and invasion/metastasis of human gastric cancer SGC-7901 cells

背景与目的:人胃癌组织微小RNA表达谱芯片筛选研究报道miR-1284与胃癌淋巴结转移有关,但其在胃癌中的具体作用鲜见报道。该研究探讨miR-1284过表达对人胃癌SGC-7901细胞基因表达谱及侵袭转移的影响。方法:以慢病毒介导miR-1284过表达转染胃癌SGC-7901细胞为实验组(LV-miR-1284组),转染空载体慢病毒载体的细胞为阴性对照组(LV-NC-GFP组),未转染慢病毒载体的细胞为空白组(Control组)。采用荧光定量PCR检测各组细胞miR-1284的表达,芯片检测各组细胞差异表达基因,生物信息学预测miR-1284的靶基因,Transwell侵袭实验检测各组细胞侵袭能力,裸鼠皮下移植瘤模型检测各组细胞转移能力。结果:PCR结果显示,与LV-NC-GFP组和Control组比较,LV-miR-1284组细胞miR-1284表达明显增高;芯片结果显示,LV-miR-1284组有20个基因表达显著上调,17个基因表达显著下调;生物信息学预测miR-1284的靶基因有138个基因;Transwell侵袭实验结果显示,LV-miR-1284组侵袭细胞数为70.00±2.37,其细胞侵袭能力较LV-NCGFP组(168.67±4.55)和Control组(170.33±3.08)明显减弱;体内裸鼠实验结果显示,LV-miR-1284组肝脏转移率为14.29%,其细胞转移能力较LV-NC-GFP组(85.71%)和Control组(85.71%)明显减弱。结论:miR-1284过表达能抑制人胃癌SGC-7901细胞侵袭转移,其机制可能与调控SUMO1、JUN基因表达有关。

Background and purpose： It has been reported that miR-1284 is associated with gastric cancer lymph node metastasis in the research of microRNA microarray in human gastric cancer tissues. But the specific role of miR-1284 in gastric cancer has not been reported. The aim of this study was to investigate the effect of miR-1284 over-expression on the gene expression profiling and invasion/metastasis of human gastric cancer SGC-7901 ceils. Methods： Gastric cancer SGC-7901 ceils of LV-miR-1284 group were transfected with lentiviral vectors of miR-1284, cells of LV-NC-GFP group were transfected with lentiviral vectors without miR-1284, and cells of control group were not transfected with lentiviral vectors. The expression of miR-1284 was detected by the real-time fluorescent quantitative PCR. Differential expression genes were detected by the microRNA chip. Target genes of miR-1284 were predicted by the bioinformaties. Invasive ability was detected by the Transwell invasion assay. Metastasis ability was detected by subcutane- ously transplanted tumor model of nude mice. Results： Compared with LV-NC-GFP and control groups, the expressions of miR-1284 and 20 genes were up-regulated, and the expression of 17 genes was down-regulated in LV-miR-1284 group. One hundred and thirty-eight target genes of miR-1284 were predicted by the bioinformatics website. Compared with invasive cell number of LV-NC-GFP group （168.67 ± 4.55） and control group （170.33 ± 3.08）, the ability of invasion of cells was weakened in LV-miR-1284 group （70.00 ± 2.37）. Compared with the liver metastasis rate of LV-NC-GFP group （85.71%） and control group （85.71%）, the ability of metastasis of ceils was weakened in LV-miR-1284 group （14.29%）. Conclusion： The ability of invasion and metastasis of SGC-7901 ceils is suppressed by over-expression of miR-1284. The mechanism may be related to regulating the expression of SUMO1 and JUN genes.