粘杆菌素流加补糖发酵工艺研究

Study on fermentation process of colistin by flow feed in sugar

粘杆菌素发酵老工艺发酵周期短,发酵单位比较低.研究了流加补糖新工艺,通过发酵过程流加葡萄糖,解除了物料浓度过高抑制粘杆菌素合成速率的问题.同时结合对发酵接种量、培养温度等参数的优化,使粘杆菌素发酵单位由110 000U/mL提高至520 000U/mL,并把该工艺成功地运用到生产中.

The old fermentation process of colistin has the disadvantages of short fermentation period and low fermentation unit.This paper developed a new sugar supplement test for colistin and studied the fed sugar fermentation process of colistin.By using fed glucose fermentation process,the inhibition of colistin synthesis rate due to the high glucose concentration can be relieved.Combined with the optimization of fermentation inoculum and culture temperature parameter,the colistin fermentation unit can go up from110 000U/mL to 520 000U/mL.And this technology has been successfully applied to large-scale production.