芎芷地龙汤对偏头痛风热证模型NO、NOS、cGMP的影响

Effect of Xiongzhi Dilong Decoction on NO,NOS and c GMP in Migraine Model with Wind-heat Syndrome

目的探讨芎芷地龙汤对偏头痛风热证一氧化氮(NO)、一氧化氮合酶(NOS)、环鸟苷酸(cGMP)的影响。方法使用热风及硝酸甘油协同诱导建立大鼠偏头痛风热证模型,观察2 h内搔头次数,用放免法测定血浆中环鸟苷酸的含量,用硝酸还原酶法测定血浆中一氧化氮的含量,用比色法测定血浆中一氧化氮合酶的含量。结果偏头痛模型组血浆NOS、NO、cGMP含量升高,与正常组、风热组相比差异有统计学意义(P<0.01);偏头痛风热证组血浆NOS、NO、cGMP含量继续升高,与正常组比较有统计学意义(P<0.01);与偏头痛模型组比较有统计学意义(P<0.05);经过治疗后血浆NOS、NO、cGMP含量恢复,与偏头痛风热证组及偏头痛模型组相比差异有统计学意义(P<0.05)。结论风热对正常机体无影响,而对硝酸甘油致偏头痛模型病理状态有显著影响,偏头痛模型受风热外袭后血浆NOS、NO、cGMP含量持续升高,芎芷地龙汤能明显改善偏头痛风热证动物模型血浆中升高的NOS、NO、cGMP含量。

Objective To study the effects of Xiongzhi Dilong decoction（XDD） on nitric oxide （NO）/NO synthase （NOS）/cyclic guanosine monophosphate （cGMP） in migraine with wind - heat syndrome.Methods The migraine model with wind - heat syndrome was established by combining exterior pathogen（hot wind） and GTN administration.The frequency of scratching head within 2 h was examined.The contents of plasma cyclic cGMP, NO and NOS were assayed by radioimmunoassay（RIA）, nitrate reductase method and colorimetric method.Results The contents of plasma NOS, NO,and cGMP in migraine model were significantly higher than that in normal group and wind - heat group（ P 〈0.01） ,and the contents of plasma NOS,NO and cGMP in migraine with wind - heat syndrome model persistently increased,which was significant difference compared to normal group（ P 〈0.01）, and was significant difference compared to migraine model group（ P 〈0.05）.The contents of plasma NOS, NO and cGMP in Chinese medicine treatment group were recovered, which was significant difference compared to migraine with wind heat syndrome model and migraine model group（ P 〈0.05）.Conclusion There is no significant effect on normal organism which sufferd from wind heat,but there is significant effect on migraine model induced by nitroglycerin.The content of plasma NOS, NO and cGMP in migraine model which is invaded by wind and heat persistently increased.XDD can attenuate remarkably the contents of plasma NOS, NO and cGMP in migraine model with wind - heat syndrome.