乌司他丁早期干预外源性急性肺损伤后细胞因子变化的研究

Changes of cytokines in exogenous acute lung injury after early intervention with Ulinastatin in New Zealand rabbits

目的:通过建立新西兰兔的急性肺损伤模型,观察乌司他丁干预后的IL-4、IL-10、IL-13浓度水平的变化。方法:将新西兰兔建模后,随机分为对照组及实验组。对照组新西兰兔致伤后注入5 m L生理盐水,实验组新西兰兔致伤后注入5 m L(2万U/kg)乌司他丁。在伤前及伤后固定时相点抽新西兰兔动脉血做血气分析并检测炎性因子;处死实验兔,并取肺部组织标本于光镜下行病理学检查。结果:外源性急性肺损伤后对照组抗炎因子分泌不足,炎性反应明显;乌司他丁干预后能明显提升IL-4、IL-10、IL-13抗炎因子的水平,3 h后下降,但12 h后实验组抗炎因子水平仍高于对照组水平。结论 :乌司他丁能够在一定程度上降低外源性急性肺损伤的炎性反应。

Objective： Using exogenous acute lung injury model in New Zealand rabbits, the effect of Ulinastatin（UTI）on the levels of cytokines（IL-4, IL-10, and IL-13）was investigated. Methods： Model animals were randomly divided into control and experimental groups, where 5 mL physiological salient or 5 m L UTI （20 000 U/kg） was administered respectively after acute lung injury. Blood samples were collected before and at specific time points after injury, and arterial blood was used for blood gas analysis and venous blood was used to measure inflammatory factors using ELISA. After sacrifice of animals, the pathological changes of lungs were evaluated under light microscopy. Results： After exogenous acute lung injury, there was insufficient secretion of anti-inflammatory factors and marked inflammation in control animals, and UTI treatment significantly increased the secretion of IL-4, IL-10, and IL-13 at 3 and 12 hours after injury. Conclusion： UTI can ameliorate inflammation in some degree in exogenous acute lung injury.