大鼠I型肌醇三磷酸受体多种突变体的载体构建

目的构建大鼠I型肌醇三磷酸受体（IP3R1）多种突变体真核表达载体。方法以pcDNA3．0-IP3R1 WT为模板，利用重组融合的方法定点突变构建3个突变体，根据点突变的位置分别命名NG2586A、12588V、R2596A。结果构建的3个突变体，经PCR、酶切、测序，3个位点的突变完全达到预期设计。结论成功构建3个突变体，为进一步研究IP3R1对大鼠平滑肌细胞中胞浆内Ca2＋浓度的调控作用奠定基础。

Objective The different mutants of eukaryotic expression vectors of Rattus norvegicus IP3R1 were constructed Methods The pcDNA3.0-IP3R1 WT was used as a template to construct three mutants by the method of recombinant fusion, and according to the position of the point mutation, they were named as G2586A, I2588V, R2596A, respectively. Results The three mutants were confirmed by using PCR, enzyme digestion, sequencing, and achived the expected design Conclusion The three successfμ1 mutants lay the foundation for the further study on the reg μ latory effect of IP3R1 on Ca2＋ concentration in cytoplasm of smooth muscle cells.