摘要
目的对胃癌淋巴结转移相关长链非编码RNA(10ngnon—codingRNA,lncRNA)进行筛选和验证。方法应用AgilentArray平台对3例胃癌阳性淋巴结和1例正常淋巴结行lncRNA芯片,聚类分析lncRNA和mRNA表达水平检测,筛选胃癌转移淋巴结中异常表达的lncRNA。随机选取15个高表达和15个低表达的lncRNAs,应用RT-PCR验证这些lncRNAs的表达水平。结果与正常淋巴结相比.将胃癌转移淋巴结中表达变化6倍以上者确定为差异表达者,其中353个lncRNAs表达上调和464个lncRNAs下调.547个mRNAs上调和562个mRNAs下调。lit.PCR实验结果显示:与正常淋巴结相比,lncRNAOR3A4、LOC84740、FCGRlC和C210ff96在胃癌阳性淋巴结中表达上调,lncRNAMST02P、LOC344595、TUGl。TYw3和KRT8P10在胃癌阳性淋巴结中表达下调。结论lncRNAs在胃癌淋巴结转移中存在异常表达。
Objective To analyze and verify the expression profiles of long non-coding RNAs (lncRNAs) in gastric cancer (GC) metastatic lymphonodus. Methods Microarray analysis was performed in 3 GC positive lymphonodus and 1 normal lymph node with Agilent Array platform to measure the expression levels of lncRNAs and mRNAs and to investigate the expression differences of lneRNAs in GC metastatic lymphonodus and normal lymphonodus, and hierarchical clustering used to screen out the differently expressed lncRNAs. 15 up-regtdated lncRNAs and 15 down-regulated lncRNAs were randomly chosen and RT-PCR was used to verify the expression differences. Results Comparing with normal lymphonodus, 353 lncRNAs and 547 mRNAs are up-regulated, but 464 lncRNAs and 562 mRNAs are down- regulated in GC metastatic lymphanodus as 6 times or more variation was found. The expressions of lncRNA OR3A4, LOC84740, FCGRIC and C21orf 96 were increased in GC metastatic lymphonodus, but lncRNA MSTO2P, LOC344595, TUG1, TYW3 and KRTSP10 decreased. Conclusions LncRNAs are aberrantly expressed in GC metastatic lymphonodus.
出处
《中华普通外科杂志》
CSCD
北大核心
2015年第10期800-803,共4页
Chinese Journal of General Surgery
基金
国家自然科学基金青年基金资助项目(81101858、81302147)
山东省博士基金资助项目(BS2013YY045)
中国博士后基金资助项目(2014M560561)
关键词
胃肿瘤
淋巴转移
芯片分析技术
长链非编码RNAs
Stomach neoplasms
Lymphatic metastasis
Microchip analytical procedures
Long non-coding RNAs