Plackett-Burman试验联合响应面试验优选XDA-7型大孔树脂纯化白子菜总多糖的工艺条件

Optimization of Purification Technology of Total Polysaccharides from Gynura divaricata Stems and Leaves with XDA-7 Macroporous Resin by Plackett-Burman Design and Response Surface Methodology

目的:优选XDA-7型大孔吸附树脂纯化白子菜总多糖的条件,为该有效部分的充分利用提供参考。方法:采用Plackett-Burman试验筛选影响总多糖纯化工艺的显著性因素,以总多糖保留率及蛋白质去除率为因变量,上样量、流速、洗脱体积、径高比、上样液质量浓度及p H为自变量,通过响应面试验优选白子菜总多糖的纯化条件。结果:上样量、流速和洗脱体积为影响总多糖纯化效果的显著性因素,在上样量0.93 g·g-1(生药材-树脂),流速1.36 m L·min-1,洗脱剂用量4.3 BV的条件下,白子菜总多糖保留率93.08%,蛋白质去除率49.13%。二者与预测值的相对误差分别为3.49%,2.09%。结论:XDA-7型大孔吸附树脂能较好地保留总多糖、去除蛋白质,适用于白子菜总多糖的纯化处理。

Objective： To optimize purification technology of total polysaccharides from Gynura divaricata stems and leaves by XDA-7 macroporous resin. Method： Significant factors affecting purification of total polysaccharides were firstly screened by Plackett-Burman test,taking retention rate of total polysaccharides and removal rate of protein as indexes,response surface analysis was adopted to optimize purification technology of total polysaccharides with flow rate, elution volume, ratio of diameter to height, sample volume, sample liquid concentration and p H as independent variables. Result： Sample volume, elution volume and flow rate were significant factors affecting purification effect of total polysaccharides. Optimum technology conditions were as follows： sample volume（ G. divaricata as crude drug）-resin volume ratio of 0. 93 g·g-1,washed with 4. 3 BV of water as eluant,elution flow rate of 1. 36 m L·min-1. Retention rate of total polysaccharides and removal rate of protein were 93. 08% and 49. 13%, respectively. Conclusion： XDA-7 macroporous resin has good effect for retention of total polysaccharides and removing protein,while serving as bleaching effect,which indicates that this method can well purity total polysaccharides in G. divaricata stems and leaves.