响应面法优化白花蛇舌草中齐墩果酸和熊果酸的提取工艺

Optimization of Extraction Process of Oleanolic Acid and Ursolic Acid from Hedyotis diffusa by Response Surface Methodology

目的:优选白花蛇舌草中齐墩果酸和熊果酸的提取工艺,为该药材的资源开发提供参考。方法:在单因素试验基础上,以齐墩果酸和熊果酸提取量的综合评分为指标,根据Box-Benhnken中心组合设计原理,利用响应面分析法考察料液比、提取次数、提取时间对白花蛇舌草中齐墩果酸和熊果酸提取工艺的影响。结果:最佳提取工艺为加10.5倍量85%乙醇加热回流提取2次,每次92 min。齐墩果酸、熊果酸提取量分别为0.497 6,2.036 4 mg·g-1,综合评分97.23分,与预测值(99.05分)的相对误差1.8%。结论:通过响应面法优选的白花蛇舌草提取工艺合理可行,提取率高,易于工业化大生产,为该药材的开发应用提供实验依据。

Objective： To optimize extraction process of oleanolic acid and ursolic acid from Hedyotis diffusa. Method： On the basis of single factor tests, according to Box-Behnken design principles, response surface analysis was adopted to investigate effects of solid-to-liquid ratio, extraction times and time on comprehensive score of extracting amounts of oleanolic acid and ursolic acid from H. diffusa. Result： The best extraction process of oleanolic acid and ursolic acid was adding 10. 5 times the amount of 85% ethanol,reflux extracting for twice,92 minutes for each time. Yield of oleanolic acid and ursolic acid were 0. 497 6 mg·g-1and2. 036 4 mg·g-1,respectively; comprehensive score was 97. 23 points,its relative error was 1. 8% by comparing with the predicted value. Conclusion： This process is reasonable and feasible with high extraction rate,and it is suitable for industrial production. This study can provide experimental basis for further development and application of H. diffusa.