何首乌中核苷类成分QTRAP UPLC-MS/MS分析

Analysis of Nucleosides and Nucleobases in Polygoni Multiflori Radix by UPLC-MS / MS

目的:建立同时测定何首乌中10种核苷类成分的QTRAP UPLC-MS/MS分析方法,分析比较生何首乌和制何首乌中核苷类成分的差异。方法:采用QTRAP UPLC-MS/MS技术,Waters Atlantis T3色谱柱(2.1 mm×150 mm,3μm),流动相甲醇-5 mmol·L-1乙酸铵(含0.1%冰乙酸),0.4 m L·min-1梯度洗脱,采用正离子多反应监测模式测定何首乌商品药材中10种核苷类成分的含量。结果:10种核苷类成分具有良好的线性关系,相关系数均>0.99;何首乌商品药材中核苷类成分尿苷、腺嘌呤、鸟苷、胞苷含量均较高;生何首乌与制何首乌中核苷类成分含量差异明显。结论:该方法简便、灵敏、准确。该研究为何首乌药材内在质量的评价和控制提供可靠的检测方法。

Objective： To develop a UPLC-QTRAP-MS /MS analysis method for the simultaneous determination of 10 nucleosides and nucleobases in Polygoni Multiflori Radix,and analyze the differences of nucleosides and nucleobases in raw and prepared Polygoni Multiflori Radix. Method： QTRAP UPLC-MS / MS technology was performed on a Waters Atlantis T3column（ 2. 1 mm × 150 mm,3 μm）,eluted by a mobile phase of methanol-5 mmol·L-1ammonium acetate（ 0. 1% acetic acid） at a flow rate of 0. 4 m L ·min-1. Ten kinds of nucleosides and nucleobases were analyzed in the positive ion multiple reaction monitoring（ MRM） mode. Result：Ten nucleosides and nucleobases had good linearities,correlation coefficient 〉0. 99; the content of uridine,adenine,guanosine and cytidine was higher in Polygoni Multiflori Radix; there was significant difference in nucleoside compositions between raw and prepared Polygoni Multiflori Radix. Conclusion： The method is simple,sensitive,accurate,and can provide a reliable and effective technique for the quality control of Polygoni Multiflori Radix.