鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较

Comparison of HPLC Fingerprint of Flavonoid in Dry and Fresh Spatholobi Caulis

目的:建立鸡血藤黄酮部位的HPLC指纹图谱,通过比较同一产地鸡血藤干、鲜品药材的指纹图谱,考察两者化学成分种类和含量上的差异。方法:采用高效液相色谱法,DiamonsilC18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸溶液为流动相梯度洗脱,流速1.0 m L·min-1,检测波长280 nm,检测时间80 min。结果:分别建立了干、鲜鸡血藤黄酮部位的HPLC指纹图谱共有模式,标定了11个共有指纹峰,并指认了4个共有峰,发现干、鲜品在成分和含量上均有差异。结论:该方法简便、准确,具有良好的重复性,可为鸡血藤化学成分深入研究及其加工利用提供科学依据。

Objective： To establish the HPLC fingerprint of flavonoid in Spatholobi Caulis,and observe the difference in chemical compositions and content between dry and fresh Spatholobi Caulis from the same origin by comparing the fingerprint of them. Method： HPLC method was adopted. The determination was performed on Diamonsil  C18column（ 4. 6 mm × 250 mm,5 μm） with acetonitrile-0. 2% phosphoric acid water solution as eluent gradient at the flow rate of 1. 0 m L·min-1. The detection wavelength was 280 nm. The detection time was80 min. Result： The common mode of HPLC fingerprint of flavonoid in dry and fresh Spatholobi Caulis was set up respectively. There were 11 common peaks in the fingerprint of dry and fresh herbs. Four of them were recognized.Conclusion： The method is simple,accurate and has a good repeatability. It can provide scientific basis for processing and utilization of Spatholobi Caulis.