柴胡疏肝散对肝郁证大鼠血清MOT以及ICC-DMP细胞的影响

Effect of Chaihu Shugan San on Serum MOT and ICC-DMP in Rat with Liver Depression Syndrome

目的:研究柴胡疏肝散对肝郁证模型大鼠血清胃动素(MOT)以及胃肠道Cajal间质细胞深肌丛(ICC-DMP)的影响。方法:选取50只清洁级SD大鼠,随机分为正常组、造模组、柴胡疏肝散高、低剂量组(21.0,10.5 g·kg-1,ig)、吗丁啉组(10 mg·kg-1,ig),每组10只。除空白组,其余4组均采用慢性应激刺激造模法进行为期3周的造模。造模后,按各组剂量每天10 m L·kg-1ig给药2周,空白组及造模组均ig给以等体积生理盐水。观察体重及强迫游泳不动时间的改变,酶联免疫吸附法(ELISA)检测大鼠血清MOT的水平,并在电镜下观察ICC-DMP的变化。结果:与正常组比较,造模后模型组大鼠体重均明显减轻;强迫游泳不动时间明显延长(P<0.05);MOT分泌明显减少(P<0.05)。给药后,与模型组比较,柴胡疏肝散能显著减少强迫游泳不动时间(P<0.05);显著升高血清MOT水平(P<0.05),电镜观察ICC-DMP,其结构和功能也有明显改善。结论:柴胡疏肝散具有促进胃肠道动力作用,其机制可能与其调节肝郁大鼠血浆MOT的水平以及调节ICC-DMP数量及功能有关。

Objective： To study the effect of Chaihu Shugan San（ CHSGS） on serum motilin（ MOT）and interstitial cells of Cajal-deep muscular plexus（ ICC-DMP） in model rats with liver depression syndrome.Method： All 50 SD rats were randomly divided into 5 groups： normal group,model group,the high and low dose groups of CHSGS（ 21. 0,10. 5 g·kg-1,ig）,and domperidone group（ 10 mg·kg-1,ig）,10 rats in each group.The rats in rest 4 groups except normal group received modeling for 3 weeks by chronic unpredicted mild stress（ CUMS）. In the next 2 weeks after stimulus,each group was treated with gavage intervention daily 10 m L·kg-1.The normal and the model group received ig normal saline（ NS） of the same volume. The changes of weight and ethological indexes of immobility time in forced swimming test were observed before and after the administration of CHSGS. Serum MOT levels were detected by using enzyme linked immunosorbent assay（ ELISA）,and ICC-DMP changes were observed under electron microscopy. Result： Compared with the normal group,the rats in model group had an obvious weight loss after modeling; immobility time was prolonged（ P〈0. 05）; serum MOT levels were decreased（ P〈0. 05）. After administration, compared with model group, CHSGS could reduce the immobility time in forced swimming test（ P〈0. 05）,significantly increase serum MOT levels（ P〈0. 05）,and significantly improve the structure and function of ICC-DMP under electron microscope. Conclusion： CHSGS has the effect of promoting gastrointestinal motility,and the mechanism may be associated with regulating serum MOT levels,as well as quantity and function of ICC-DMP in rats with liver depression syndrome.