GPV感染雏鹅的保护酶活性与同工酶变异性研究

The isozyme variability and activity of protective enzymes from goslings infected goose parvovirus

酶是催化代谢途径的高效活性基因产物,应激环境和遗传基础的不同导致同工酶结构或活性差异,使不同组织和发育阶段的酶种类和活性表现特异性变化。采用生化分析实验技术和PAGE分析GPV感染雏鹅保护酶（POD、ES、SOD）的活性及同工酶特征变异,结果表明,GPV感染雏鹅的脑、心、脾组织新出现1条POD同工酶带,肺组织消减1条同工酶带;GPV感染雏鹅的肝、肺、心组织SOD同工酶谱带新增加2条,脑、脾组织新增加1条,脑、心、脾分别缺少慢区、中区、快区酶带;GPV感染雏鹅的心、脾组织ES同工酶分别出现2、3条新酶带,脑、肝、肺组织新出现1条酶带;POD、SOD、ES活性不同程度分别增强1.2～6倍、1～1.7倍、1～2倍,肝中最显著（6,1.7,2倍）。提示GPV感染应激与寄主保护酶基因表达的互作作用,引起酶的谱型、活性等生化特征变异,直接或间接调控代谢途径与病理生化性能,因此,保护酶是GPV感染应激的敏感酶,可作为GPV与宿主易感性互作致病机制研究的有效标记。

The enzyme is efficiently active gene product catalyzed metabolic pathway.Distinct environmental and genetic basis of stress leads to a difference of isozyme structure or activity in different tissues and developmental stages.Here,the peroxidase（POD）,esterase（ES）and superoxide dismutase（SOD）were respectively extracted from brain,heart,iver,lung,spleen organ of goslings infected goose parvovirus and control group.The results of PAGE and biochemical analysis showed that there appeared 1new band of POD isozyme in the brain,heart and spleen of goslings infected goose parvovirus,but deleted 1band of POD isozyme in the lung of goslings infected goose parvovirus.There were 2new SOD isozyme bands in liver,lung and heart of goslings infected goose parvovirus,and 1new SOD isozyme band in brain and spleen,but deleted slow-zone,slow-medium and fast-zone SOD isozyme bands in the brain,heart and spleen of goslings infected goose parvovirus.There were 2and 3new ES isozyme bands in heart and spleen respectively,and1 fast new ES isozyme band in brain,liver and lung in goslings infected goose parvovirus.The activity of peroxidase,esterase and superoxide dismutase from goslings infected goose parvovirus were higher 1.2-6times,1-1.7times,1-2times than that of control group respectively,and were the greatest in the liver of goslings infected goose parvovirus.Those indicated that the interaction of GPV stress and host protective enzyme gene expression resulted in the biochemical characteristics variation of activity and isozyme patterns of protective enzyme,and directly or indirectly affected metabolic approach and pathological biochemical function.Therefore,protective enzyme might be sensitive enzyme to GPV infection stress,and were effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.