Myc结合的锌指蛋白1评估实验性急性胰腺炎疾病严重程度的价值

Significance of MIZ1 expression on severity of experimental acute pancreatitis

目的检测急性坏死性胰腺炎（ANP）大鼠血清及胰腺组织Myc结合的锌指蛋白1（MIZ1）表达，探讨其评估急性胰腺炎（AP）病情严重程度的价值。方法采用胰管逆行注射牛磺胆酸钠方法制备ANP模型。以注射生理盐水作为对照组。术后6、24、48h处死大鼠，取血及胰腺组织。采用ELISA法检测血清淀粉酶活性及C反应蛋白（CRP）、TNF-α、IL-6、MIZ1水平。常规行胰腺组织病理学检查，并采用免疫组化及蛋白质印迹法检测胰腺组织MIZ1蛋白表达。结果对照组及ANP6、24、48h组大鼠血清淀粉酶活性分别为（449±40）、（578±25）、（1021±205）、（971±143）U／L；CRP水平为（123±23）、（169±25）、（226±34）、（229±24）mg／L；IL-6为（20．16±4．11）、（38．60±12．05）、（52．33±6．77）、（44．83±4．30）ng／L；TNF-α为（55．334-3．32）、（82．8±5．26）、（120．66±16．00）、（108．33±12．17）ng／L；MIZ1为（5．51士0．34）、（3．44±0．56）、（2．11±0．11）、（2．41±0．43）ng／L；胰腺病理评分为（1．83±0．75）、（6．00±1．67）、（8．16±2．70）、（9．33±1．50）分；胰腺组织MIZ1表达量为0．81±0．05、0．53±0．07、0．31±0．06、0．21±0．08。除ANP6h组的血淀粉酶活性外，其他所有指标ANP各组与对照组差异均有统计学意义（P值均〈0．01），ANP24、48h组又与6h组差异有统计学意义（P值均〈0．01），而ANP24h组与48h组间的差异均无统计学意义。血清MIZl水平与血清淀粉酶、CRP、TNF-α、IL-6水平及胰腺组织病理学评分均呈负相关，差异均有统计学意义（P值均〈0．01）。结论MIZl在AP病程中低表达，并与病情严重程度密切相关，可作为评估AP预后的指标之一。

Objective To explore the relationship between the severity of experimental acute pancreatitis （AP） and the expression of Myc interacting zinc finger protein 1 （MIZ1） in rat, to evaluate the value of MIZ1 for severity assessment. Methods Acute neerotizing pancreatitis（ANP） model was induced by retrograde injection of sodium taurocholate into the pancreatic duct, and normal saline was used in control group. The rats were sacrificed at 6, 24, 48 h, and the blood and pancreas tissue was collected, and serum amylase level, C reactive protein （CRP）, TNF-α, IL-6 and MIZ1 were determined by ELISA. Pancreatic tissue was routinely examined by pathologist, and the MIZ1 protein expression in pancreatic tissue was measured by immunohistoehemistry and Western blot. Results The serum amylase levels of control group and ANP group at 6, 24, 48 h were （449±40）, （578±25）, （1 021±205）, （971 ± 143） U/L, and the levels of CRP were （123 ± 23 ）, （169 ±25 ）, （226 ± 34）, （229 ±24）mg/L; and the levels of IL-6 were （20.16 ± 4.11）, （38.60 ±12.05）, （52.33 ±6.77）, （44.83 ±4.30）rig/L; and the levels of TNF-et were （55.33 ± 3.32）, （ 82.8 ± 5.26）, （ 120.66 ± 16.00）, （ 108.33 ± 12.17） rig/L; and the levels of MIZ1 were （ 5.51 ± 0.34） , （3.44 ± 0.56） , （2.11 ±0.11 ） , （2.41 ± 0.43） ng/L. The pathologic scores of pancreas were （ 1.83 ±0.75）, （6.00 ± 1.67）, （8.16 ±2.70）, （9.33 ± 1.50）, and the expressions of MIZ1 in pancreatic tissue were 0.81 ±0.05, 0.53 ± 0.07, 0.31 ± 0. 06, 0.21 ± 0.08. Except for amylase level of ANP 6h group, other parameters of ANP group were significantly different with those of control group, and the parameters of ANP 24, 48 h group were significantly different with that of ANP 6 h group （ P 〈 0.01 ） , but there was no significantly different between ANP 24 and 48 h group. MIZ1 expression was negatively correlated with serum amylase level, CRP, TNF-α, IL-6 and pathologic scores of pancreas, and the difference was statistically signific ant （P 〈0.01 ）. Conclusions The decreasing expression of MIZ1 is closely correlated with the severity of AP, and may be a potential marker for prognosis evaluation.