‘黄水蜜’桃实生苗茎段再生体系的建立

Establishment of regeneration system for stem segments from seedlings of‘Huangshuimi'

【目的】建立‘黄水蜜’桃实生苗茎段高效稳定的再生体系。【方法】以‘黄水蜜’桃实生苗茎段为外植体,探讨了不同植物生长调节剂质量浓度组合对不定芽诱导过程中愈伤组织形成率和不定芽形成率的影响,并采用两步生根法研究不同植物生长调节剂种类对不定根诱导的影响。【结果】适合‘黄水蜜’桃实生苗茎段切口处愈伤组织再生的最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1+Ag NO30.5 mg·L-1,不定芽再生的最佳培养基为MS+6-BA 3.0mg·L-1+NAA 0.1 mg·L-1+Ag NO30.5 mg·L-1,其愈伤组织形成率和不定芽再生率分别为85%和47%;获得的不定芽先后经WPM+ZT 2.0 mg·L-1+NAA 0.1 mg·L-1+Ag NO30.5 mg·L-1和MS+NAA 0.5 mg·L-1+GA30.5 mg·L-1+Ag NO30.5mg·L-1两步生根法培养后,不定根再生率为50%,平均根数量为5.00。【结论】初步建立了‘黄水蜜’桃实生苗茎段再生体系,为桃遗传转化研究奠定基础。

【Objective】The objective of the study is to establish the stable regeneration system from stem segments of‘Huangshuimi＇seedlings.【Methods】The effects of different plant growth regulators on frequency of callus formation and adventitious shoot regeneration were investigated by using stem segments of‘Huangshuimi＇seedlings as explants. Moreover,the influence of different types of plant growth regulator on the induction of adventitious root was analyzed by using two-step rooting method.【Results】The results indicated that the optimal medium for callus induction from stem segments was MS containing 2.0mg·L^-16-BA,0.5 mg·L^-1NAA and 0.5 mg·L^-1Ag NO3,while for adventitious shoot induction was MS supplemented with 3.0 mg·L^-16-BA,0.1 mg·L^-1NAA and 0.5 mg·L^-1Ag NO3. The frequency of callus formation and frequency of adventitious shoot regeneration were 85% and 47%,respectively. The adventitious shoots were cultured in WPM including 2.0 mg·L^-1 ZT,0.1 mg·L^-1 NAA and 0.5 mg·L^-1 Ag NO3,then transferred to the MS supplemented with 0.5 mg·L^-1NAA,0.5 mg·L^-1 GA3 and 0.5 mg·L^-1 Ag NO3.The rate of adventitious root regeneration and the average number of roots were 50% and 5.00,respectively.【Conclusion】An efficient regeneration system from stem segments of‘Huangshuimi＇seedlings which lays the foundation for the genetic transformation of peach was established in this study.