白腐真菌协同超声酵解浸提废次烟叶中茄尼醇

Extraction process of solanesol from zymolytic tobacco waste by combining white rot fungus with ultrasonic

【目的】研究提取废次烟叶中茄尼醇的白腐真菌与超声协同作用,减少茄尼醇溶出阻力,为废次烟草减量化与资源化利用提供技术支持。【方法】以废次烟叶为原料,采用单因素试验考察茄尼醇溶出过程中酵解时间、p H、烟叶质量与水体积比、水浴温度、发酵液量,以及浸提过程中超声时间、料液比等因素对茄尼醇溶浸效果的影响,并对比不同浸提方法的提取效果;同时以红外光谱图解析烟叶植物组织结构及其表壁官能团变化,采用高效液相色谱法分析测定茄尼醇质量浓度。【结果】白腐真菌酵解后烟叶表面C-O-C、-CH3和C=O等特征吸收峰明显;白腐真菌协同超声酵解浸提茄尼醇的适宜参数为:烟叶质量与水体积比1∶20、白腐真菌发酵液接入量1.5 m L、水浴温度30℃、p H 7、酵解时间12 h、料液比1∶20、超声波功率200 W、超声时间25 min,在此条件下茄尼醇质量浓度达0.240 g/L,浸提率为97.88%,是超声浸提的1.31倍,是传统化学醇提法的1.76倍。【结论】白腐真菌有助于降解烟叶植物组织中C-O-C、-CH3和C=O,白腐真菌协同超声酵解浸提法可有效提取废次烟叶中茄尼醇。

【Objective】The synergistic effect between white rot fungus and ultrasonic on extraction of solanesol from waste tobacco was investigated to decrease resistance to dissolving out solanesol from waste tobacco, in order to provide technical support for reducing and utilizing waste tobacco. 【Method】The zymolytic time, p H, tobacco mass/water volume ratio, bathing temperature, fermentation broth volume, ultrasonic time and material-liquid ratio（tobacco mass/methanol volume ratio） was taken as main influence factors for dissolving out and extracting solanesol. Using waste tobacco as materials, the influence of these factors on dissolving and extracting effects of solanesol were analysed to compare different extracting method based on single-factor test. Meanwhile, the tobacco tissue structure and its external wall＇s functional groups were analyzed on the basis on infrared spectra figures. The content of solanesol was detected by using HPLC method. 【Result】The results showed that there were characteristic absorption peaks of functional group viz., C-O-C,-CH3 and C=O to be found on the external wall of tobacco fermented by white rot fungus. The optimal extraction process parameters of solanesol from zymolytic tobacco by combining white rot fungus and ultrasonic were as follows： tobacco mass/water volume raito of 1∶20, 1.5 m L fermentation broth of white rot fungus, bathing temperature of 30 ℃, p H 7.0, zymolytic time of 12 h, material-liquid ratio of 1 ∶20, ultrasonic power of 200 W, ultrasonic time of 25 min. Under the above optimum conditions, the mass concentration of solanesol reached up to 0.240 g/L, and the extraction rate of solanesol was 97.88%,which was 1.31 times of that under conditions of ultrasonic extraction, and 1.76 times of that under conditions of chemical extraction. 【Conclusion】White rot fungus can significantly promote degradation of C-O-C,-CH3 and C=O in tobacco tissue. Furthermore, the extraction process for combining white rot fungus and ultrasonic will be effective to extract solanesol from waste tobacco.