湖南汉族系统性红斑狼疮患者FcγRⅢa—158V/F多态性研究

Polymorphism of Fc Gamma Receptor Ty peⅢin Han Patients with Systemic Lupus Erythematosus from Hunan Province

目的探讨中国湖南汉族SLE患者与FcγRⅢa—158V/F多态性的相关性。方法SLE患者65例,其中狼疮肾炎患者38例,采用多聚酶链反应(PCR)和限制性片段长度多态性分析法(RFLP)检测FcγRⅢa—158V/F基因型,并与60例正常人进行比较。结果①与对照组相比,SLE患者FcγRⅢa—158F/F纯合子基因型显著增高(OR=2.23,χ2=4.69,P=0.03)。②与对照组相比,狼疮肾炎患者Fc-γRⅢa—158F/F纯合子基因型和FcγRⅢa—158F等位基因频率均显著增高(OR=2.67,χ2=5.36,P=0.02;OR=2.00,χ2=4.91,P=0.03)。结论SLE患者FcγRⅢa—158F/F纯合子基因型显著增高,狼疮肾炎患者FcγRⅢa—158F/F纯合子基因型和FcγRⅢa—158F等位基因频率均显著增高,提示FcγRⅢa多态性与SLE相关,FcγRⅢa—158F等位基因可能是狼疮肾炎的危险因素。

Objective To investigate the association betw een systemic lupus erythematosus(SLE)and polymorphismof Fc gamma receptor ty peⅢin Han patients fromHunan province.Methods Genotypes of FcγRⅢa-158V/F were determined by polymerase chain reaction(PCR)and restriction fragment length polymorphism(RFLP)analysis in 65patients with SLE and 60normal controls.Results①It was found that the frequency of homozygous FcγRⅢa-158F /F genotype was significantly h igher in patients with SLE than that i n controls(OR=2.23,χ 2 =4.69,P=0.03).②The frequencies of both homozygous FcγRⅢa-158F /F genotype and FcγRⅢa-158F allele were significantly high er in patients with lupus nephritis c ompared with those in controls(OR=2.67,χ 2 =5.36,P=0.02;OR=2.00,χ 2 =4.91,P=0.03).Conclusions These results suggest that an abnorm al distribution of FcγRⅢa-158V/F polymorphism is associated with SLE in the Hans of Hunan province,and the presence of FcγRⅢa-158F allele is a risk factor for lupus nephritis.These findings support t he hypothesis of a genetic mechanism in the pathogenesis of SLE.[