蕈样肉芽肿的端粒酶研究

Detection of Telomerase Activity in Patients with Mycosis Fungoides

目的检测各期蕈样肉芽肿(MF)的端粒酶活性,探讨端粒酶在MF肿瘤发生机制中的作用。方法采用端粒酶聚合酶链反应-酶联免疫吸附测定法(PCR-ELISA),对35例MF患者进行端粒酶的定性和定量分析。结果92.3%的肿瘤期MF、78.6%的斑块期MF和75.0%的斑片期MF显示端粒酶阳性;肿瘤期MF的端粒酶活性水平高于斑块期和斑片期,且差异有显著性;而斑块期与斑片期之间差异无显著性。对照组的端粒酶均为阴性。讨论MF患者存在高水平的端粒酶活性,提示端粒酶可能在MF的肿瘤发生中起重要作用,对MF的诊断有一定的临床意义。

Objective To detect telomerase activity in patients with mycosis fungoides(MF )and study the role of telomerase in the tumorigenesis of MF.Methods Polymerase chain reaction -enzyme l inked immunosor-bent assay(PCR-ELISA)was employed to detect telomerase ac tivity in 35patients with different stages of mycosis fungoides.Results Telomerase was positively detected in 92.3%patients with tumor stage,78.6%plaque stage and 75.0%patch stage of MF.Telomera se activity was significantly higher in tumor stage than that in plaque an d patch stage.No significant differe nce of telomerase activity was found between plaque stage and patch stage.No telomerase activity was detected in control group.Conclusions High level of telomerase activity is observed in MF patients,suggesting that telomera se may play an important role in the tu morigenesis of MF and may serve as a useful marker for the diagnosis of MF.[