细胞周期G1调节因子在尖锐湿疣皮损中的表达

Expression of Cell Cycle Related Factors Cyclin D1,CDK4,P16^(MTS1) and PRb in Condyloma Acuminatum

目的探讨细胞周期G1调控点(checkpoint)相关调节因子细胞周期素D1(CyclinD1)、周期素依赖性蛋白激酶4(CDK4)、多重肿瘤抑制因子(P16MTS1)及视网膜母细胞瘤蛋白(PRb)在HPV6/11相关尖锐湿疣(CA)皮损中的表达和意义。方法采用SP免疫组化染色技术检测27例经PCR检测含HPV6/11DNA的CA皮损和10例正常人皮肤(包皮)中CyclinD1、CDK4、P16MTS1及PRb的表达和分布。结果①正常人皮肤及CA皮损均无CyclinD1表达。②CA皮损基底细胞表达CDK4(均数2.19)较正常人皮肤(均数4.8)降低(P<0.01)。③CA皮损棘细胞表达P16MTS1(均数1.33)较正常人皮肤(均数0)增强(P<0.05)。④CA皮损基底细胞和棘细胞表达PRb(均数各为0.89、0.44)较正常人皮肤(均数均为0)均增强(P<0.05)。结论HPV6/11CA相关皮损的细胞周期G1调控点无明显异常,可能是这类CA皮损不易恶变的原因之一。

Objective To investigate the expression and im plication of cell-cycle related factors(Cyclin D1,CDK4,P16 MTS1 and PRb)in condyloma acuminatum(CA).Methods The expression and distribution of cyclin D1,CDK4,P16 MTS1 and PRb were detected by immunohisto chemical technique streptavidin peroxidase(SP)in 27cases of HPV6/11positive CA that were confirmed by PCR and 10cases of norm al skins(foreskins).Results①Cyclin D1was not expressed in both normal skin and lesions of CA.②The intensity of expression of CDK4was 2.19in the basal cells in the lesions of CA,which was significantly weaker than that in normal skin(4.8,P<0.01).③The intensity of expression of P16 MTS1 was 1.33in prickle cells in the lesio ns of CA,which was significantly str onger than that in normal skin(0,P<0.05).④The intensity of expression of PRb in both basal and prickle cells of CA were 0.89and 0.44,respectively,which were significantly stronger than those in normal skin(0and 0,P<0.05).Conclusion There is no significant alteration i n G1check point of cell cycle in the le sions of CArelated to HPV6/11.[