低聚合度魔芋甘露寡糖对正常小鼠肠道及微生物菌群的影响

Effects of low polymerization degree konjacmannan-oligosaccharide on intestinal and microflora of normal mice

研究以有机溶剂沉淀法制备的低聚合度魔芋甘露寡糖(LMOS)对正常小鼠肠道形态、肠道菌群的影响。取ICR小鼠50只,随机分为4组实验组和1组对照组,分别用不同剂量1,2,4,8,8g/(kg·d)的LMOS溶液和等体积的生理盐水灌胃,连续30 d后,观察比较不同LMOS添加量对小鼠肠道形态、盲肠内短链脂肪酸(SCFA)及微生物菌群的影响。实验结果表明,与对照组相比,高剂量的LMOS能显著增加小鼠盲肠内SCFA的含量,并能使肠绒毛高度增加。变性梯度凝胶电泳(DGGE)指纹图谱结果分析表明,与对照组相比,喂食高剂量LMOS能使小鼠肠道中乳杆菌等有益菌含量增加。得出LMOS能够通过促进SCFA的产生、促进乳杆菌的增殖,调节小鼠肠道健康,是一类具有开发前景的益生元。

Purpose： Low polymerization degree konjacmannan oligosaccharide （LMOS） was prepared by organic solvent precipitation. Then its effects on intestinal morphology and microflora of normal mice were investigated. Methods： 50 ICR mice were randomly divided into four experimental groups and one control group. The experimental groups were gavaged with different doses of LMOS [ 1 g/（kg·d）, 2 g/（kg·d）, 4 g/（kg· d）, 8 g/（kg· d）] while the control group were gavaged with normal saline. After 30 d, intestinal morphology was observed, and both of the cecal SCFA and microbial communities in each group were determined. Results： Compared with the control group, the cecal SCFA of treated groups significantly increased. In addition, intestinal villi height increased in the treated mice. Results from DGGE fingerprint analysis showed that, the intestinal microbial community of mice fed with high dose of LMOS were regulated significantly. Conclusions ： Except increasing the production of SCFA, LMOS can also promote the proliferation of probiotics （ such as Lactobacillus） , implying that it is one kind of favorable prebiotics.